Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages

Maria A. Schumacher; Nam K. Tonthat; Jeehyun Lee; Fernando A. Rodriguez-Castañeda; Naga Babu Chinnam; Anne K. Kalliomaa-Sanford; Irene W. Ng; Madhuri T. Barge; Porsha L R Shaw; Daniela Barillà

doi:10.1126/science.aaa9046

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages

Maria A. Schumacher^*, Nam K. Tonthat, Jeehyun Lee, Fernando A. Rodriguez-Castañeda, Naga Babu Chinnam, Anne K. Kalliomaa-Sanford, Irene W. Ng, Madhuri T. Barge, Porsha L R Shaw, Daniela Barillà

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

Original language	English
Pages (from-to)	1120-1124
Number of pages	5
Journal	Science
Volume	349
Issue number	6252
DOIs	https://doi.org/10.1126/science.aaa9046
Publication status	Published - 4 Sept 2015

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10.1126/science.aaa9046

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title = "Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages",

abstract = "Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.",

author = "Schumacher, {Maria A.} and Tonthat, {Nam K.} and Jeehyun Lee and Rodriguez-Casta{\~n}eda, {Fernando A.} and Chinnam, {Naga Babu} and Kalliomaa-Sanford, {Anne K.} and Ng, {Irene W.} and Barge, {Madhuri T.} and Shaw, {Porsha L R} and Daniela Barill{\`a}",

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doi = "10.1126/science.aaa9046",

language = "English",

volume = "349",

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journal = "Science",

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TY - JOUR

T1 - Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages

AU - Schumacher, Maria A.

AU - Tonthat, Nam K.

AU - Lee, Jeehyun

AU - Rodriguez-Castañeda, Fernando A.

AU - Chinnam, Naga Babu

AU - Kalliomaa-Sanford, Anne K.

AU - Ng, Irene W.

AU - Barge, Madhuri T.

AU - Shaw, Porsha L R

AU - Barillà, Daniela

PY - 2015/9/4

Y1 - 2015/9/4

N2 - Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

AB - Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

UR - http://www.scopus.com/inward/record.url?scp=84941365152&partnerID=8YFLogxK

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DO - 10.1126/science.aaa9046

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SN - 0036-8075

VL - 349

SP - 1120

EP - 1124

JO - Science

JF - Science

IS - 6252

ER -

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages

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Daniela Barilla

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Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages

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