Substrate distortion by a beta-mannanase: Snapshots of the Michaelis and covalent-intermediate complexes suggest a B-2,B-5 conformation for the transition state

V M A Ducros, D L Zechel, G N Murshudov, H J Gilbert, L Szabo, D Stoll, S G Withers, G J Davies

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Pages (from-to)2824-2827
Number of pages4
JournalAngewandte Chemie International Edition
Volume41
Issue number15
DOIs
Publication statusPublished - Aug 2002

Keywords

  • enzyme catalysis
  • glycosylation
  • inhibitors
  • mannosidases
  • X-ray diffraction
  • GLYCOSYL-ENZYME INTERMEDIATE
  • LONE-PAIR HYPOTHESIS
  • CRYSTAL-STRUCTURE
  • GLYCOSIDASE INHIBITORS
  • ANGSTROM RESOLUTION
  • ENDOGLUCANASE-I
  • LEAVING GROUP
  • CATALYSIS
  • CRYSTALLOGRAPHY
  • SPECIFICITY

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