Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacl-galR family of regulatory genes

G P vanWezel; J White; P Young; P W Postma; M J Bibb

Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacl-galR family of regulatory genes

G P vanWezel, J White, P Young, P W Postma, M J Bibb

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

malR of Streptomyces coelicolor A3(2) encodes a homologue of the Lacl/GalR family of repressor proteins, and is divergently transcribed from the malEFG gene cluster, which encodes components of an ATP-dependent transport system that is required for maltose utilization. Transcription of malE was induced by maltose and repressed by glucose. Disruption or deletion of malR resulted in constitutive, glucose-insensitive malE transcription at a level markedly above that observed in the parental malR(+) strain, and overproduction of MalR prevented growth on maltose as carbon source. Consequently, Main plays a crucial role in both substrate induction and glucose repression of maltose utilization. malR is expressed from a single promoter with transcription initiating at the first G of the predicted GTG translation start codon.

Original language	English
Pages (from-to)	537-549
Number of pages	13
Journal	Molecular Microbiology
Volume	23
Issue number	3
Publication status	Published - Feb 1997

Keywords

ALPHA-AMYLASE GENE
CATABOLITE REPRESSION
ESCHERICHIA-COLI
NUCLEOTIDE-SEQUENCE
BACILLUS-SUBTILIS
CLONING VECTORS
XYL REPRESSOR
DNA
EXPRESSION
PROMOTERS

Cite this

@article{88e164e2aa4f4b1cb8112f50f313936c,

title = "Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacl-galR family of regulatory genes",

abstract = "malR of Streptomyces coelicolor A3(2) encodes a homologue of the Lacl/GalR family of repressor proteins, and is divergently transcribed from the malEFG gene cluster, which encodes components of an ATP-dependent transport system that is required for maltose utilization. Transcription of malE was induced by maltose and repressed by glucose. Disruption or deletion of malR resulted in constitutive, glucose-insensitive malE transcription at a level markedly above that observed in the parental malR(+) strain, and overproduction of MalR prevented growth on maltose as carbon source. Consequently, Main plays a crucial role in both substrate induction and glucose repression of maltose utilization. malR is expressed from a single promoter with transcription initiating at the first G of the predicted GTG translation start codon.",

keywords = "ALPHA-AMYLASE GENE, CATABOLITE REPRESSION, ESCHERICHIA-COLI, NUCLEOTIDE-SEQUENCE, BACILLUS-SUBTILIS, CLONING VECTORS, XYL REPRESSOR, DNA, EXPRESSION, PROMOTERS",

author = "vanWezel, {G P} and J White and P Young and Postma, {P W} and Bibb, {M J}",

year = "1997",

month = feb,

language = "English",

volume = "23",

pages = "537--549",

journal = "Molecular Microbiology",

issn = "0950-382X",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacl-galR family of regulatory genes

AU - vanWezel, G P

AU - White, J

AU - Young, P

AU - Postma, P W

AU - Bibb, M J

PY - 1997/2

Y1 - 1997/2

N2 - malR of Streptomyces coelicolor A3(2) encodes a homologue of the Lacl/GalR family of repressor proteins, and is divergently transcribed from the malEFG gene cluster, which encodes components of an ATP-dependent transport system that is required for maltose utilization. Transcription of malE was induced by maltose and repressed by glucose. Disruption or deletion of malR resulted in constitutive, glucose-insensitive malE transcription at a level markedly above that observed in the parental malR(+) strain, and overproduction of MalR prevented growth on maltose as carbon source. Consequently, Main plays a crucial role in both substrate induction and glucose repression of maltose utilization. malR is expressed from a single promoter with transcription initiating at the first G of the predicted GTG translation start codon.

AB - malR of Streptomyces coelicolor A3(2) encodes a homologue of the Lacl/GalR family of repressor proteins, and is divergently transcribed from the malEFG gene cluster, which encodes components of an ATP-dependent transport system that is required for maltose utilization. Transcription of malE was induced by maltose and repressed by glucose. Disruption or deletion of malR resulted in constitutive, glucose-insensitive malE transcription at a level markedly above that observed in the parental malR(+) strain, and overproduction of MalR prevented growth on maltose as carbon source. Consequently, Main plays a crucial role in both substrate induction and glucose repression of maltose utilization. malR is expressed from a single promoter with transcription initiating at the first G of the predicted GTG translation start codon.

KW - ALPHA-AMYLASE GENE

KW - CATABOLITE REPRESSION

KW - ESCHERICHIA-COLI

KW - NUCLEOTIDE-SEQUENCE

KW - BACILLUS-SUBTILIS

KW - CLONING VECTORS

KW - XYL REPRESSOR

KW - DNA

KW - EXPRESSION

KW - PROMOTERS

M3 - Article

SN - 0950-382X

VL - 23

SP - 537

EP - 549

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 3

ER -