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Sunflower-based biorefinery: Poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production from crude glycerol, sunflower meal and levulinic acid

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JournalBIORESOURCE TECHNOLOGY
DateE-pub ahead of print - 18 Aug 2014
DatePublished (current) - Nov 2014
Volume172
Number of pages10
Pages (from-to)121-130
Early online date18/08/14
Original languageEnglish

Abstract

Polyhydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] production was developed in bioreactor cultures using the strain Cupriavidus necator DSM 7237 cultivated on crude glycerol, sunflower meal (SFM) hydrolysates and levulinic acid as the sole fermentation feedstocks. Bacterial growth and PHB production was influenced significantly by the free amino nitrogen and inorganic phosphorus content of the SFM hydrolysate. Fed-batch bioreactor fermentations led to the production of 27gL-1 PHB with an intracellular content of 72.9% (w/w). Continuous feeding of levulinic acid led to the production of up to 23.4gL-1 P(3HB-co-3HV) with an intracellular content of 66.4% (w/w) and a 3HV content of 22.5mol%. A maximum 3HV content of 31mol% was achieved at earlier fermentation time (53h). Thus, levulinic acid could be combined with biodiesel industry by-products for the production of high P(3HB-co-3HV) concentration, intracellular content and industrially useful 3HV content.

    Research areas

  • Bacterial bioconversion, Biodiesel industry by-products, Cupriavidus necator, Polyhydroxyalkanoates

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