Superoxide is promoted by sucrose and affects amplitude of circadian rhythms in the evening

Angela Roman-Fernandez, Xiang Li, Dongjing Deng, John William Davey, Sally James, Ian Alexander Graham, Mike Haydon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Plants must coordinate photosynthetic metabolism with the daily environment and adapt rhythmic physiology and development to match carbon availability. Circadian clocks drive biological rhythms which adjust to environmental cues. Products of photosynthetic metabolism, including sugars and reactive oxygen species (ROS), are closely associated with the plant circadian clock, and sugars have been shown to provide metabolic feedback to the circadian oscillator. Here, we report a comprehensive sugar-regulated transcriptome of Arabidopsis and identify genes associated with redox and ROS processes as a prominent feature of the transcriptional response. We show that sucrose increases levels of superoxide (O2–), which is required for transcriptional and growth responses to sugar. We identify circadian rhythms of O2–-regulated transcripts which are phased around dusk and find that O2– is required for sucrose to promote expression of TIMING OF CAB1 (TOC1) in the evening. Our data reveal a role for O2– as a metabolic signal affecting transcriptional control of the circadian oscillator in Arabidopsis.
Original languageEnglish
Article number e2020646118
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
Publication statusPublished - 9 Mar 2021

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© 2021 National Academy of Sciences. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.


  • circadian
  • superoxide
  • sugar
  • redox
  • ROS

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