Superoxide is promoted by sucrose and affects amplitude of circadian rhythms in the evening

TY - JOUR

T1 - Superoxide is promoted by sucrose and affects amplitude of circadian rhythms in the evening

AU - Roman-Fernandez, Angela

AU - Li, Xiang

AU - Deng, Dongjing

AU - Davey, John William

AU - James, Sally

AU - Graham, Ian Alexander

AU - Haydon, Mike

N1 - © 2021 National Academy of Sciences. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

PY - 2021/3/9

Y1 - 2021/3/9

N2 - Plants must coordinate photosynthetic metabolism with the daily environment and adapt rhythmic physiology and development to match carbon availability. Circadian clocks drive biological rhythms which adjust to environmental cues. Products of photosynthetic metabolism, including sugars and reactive oxygen species (ROS), are closely associated with the plant circadian clock, and sugars have been shown to provide metabolic feedback to the circadian oscillator. Here, we report a comprehensive sugar-regulated transcriptome of Arabidopsis and identify genes associated with redox and ROS processes as a prominent feature of the transcriptional response. We show that sucrose increases levels of superoxide (O2–), which is required for transcriptional and growth responses to sugar. We identify circadian rhythms of O2–-regulated transcripts which are phased around dusk and find that O2– is required for sucrose to promote expression of TIMING OF CAB1 (TOC1) in the evening. Our data reveal a role for O2– as a metabolic signal affecting transcriptional control of the circadian oscillator in Arabidopsis.

AB - Plants must coordinate photosynthetic metabolism with the daily environment and adapt rhythmic physiology and development to match carbon availability. Circadian clocks drive biological rhythms which adjust to environmental cues. Products of photosynthetic metabolism, including sugars and reactive oxygen species (ROS), are closely associated with the plant circadian clock, and sugars have been shown to provide metabolic feedback to the circadian oscillator. Here, we report a comprehensive sugar-regulated transcriptome of Arabidopsis and identify genes associated with redox and ROS processes as a prominent feature of the transcriptional response. We show that sucrose increases levels of superoxide (O2–), which is required for transcriptional and growth responses to sugar. We identify circadian rhythms of O2–-regulated transcripts which are phased around dusk and find that O2– is required for sucrose to promote expression of TIMING OF CAB1 (TOC1) in the evening. Our data reveal a role for O2– as a metabolic signal affecting transcriptional control of the circadian oscillator in Arabidopsis.

KW - circadian

KW - superoxide

KW - sugar

KW - redox

KW - ROS

U2 - 10.1073/pnas.2020646118

DO - 10.1073/pnas.2020646118

M3 - Article

SN - 0027-8424

VL - 118

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 10

M1 - e2020646118

ER -