Abstract
Background: Although vitamin B-12 (B-12) is known to contribute to the structural and functional development of the brain, it is unclear if B-12 supplementation has any beneficial effect in healthy populations in terms of enhanced neurological status of the brain or improved cognitive function.
Objectives: We investigated the effect of dietary supplementation of B-12 on the cortical neural activity of well-nourished young adult rats and tested the hypothesis that B-12 supplementation in healthy rats may reduce sensory evoked neural activity due to enhanced inhibition.
Methods: Female Lister Hooded rats weighing between 190g to 265g (2 to 4 months old) were included in the study. The experimental group was fed with B-12 (Cyanocobalamin) enriched water at a concentration of 1mg/L, and the control (CON) group with tap water for 3 weeks. Animals were then anaesthetised and cortical neural responses to whisker stimulation were recorded in vivo using a multi-channel micro-electrode, from which local field potentials (LFPs) were extracted.
Results: Somatosensory-evoked LFP was enhanced 25.2% in the B-12 group (4.13±0.24mV) compared with the CON group (3.30±0.21mV) (P=0.02). However no significant difference in the frequency spectrum of the spontaneous neural activity was found between the groups, as the unpaired t-statistic at each frequency bin of interest did not pass the cluster-forming threshold of 0.05.
Conclusions: These findings do not provide evidence supporting the hypothesis of decreased neural activity due to B-12 supplementation. As the spontaneous neural activity was unaffected, the increase in somatosensory evoked LFP may be due to enhanced afferent signal reaching the barrel cortex from the whisker pad, indicating that rats supplemented with B-12 may have enhanced sensitivity to sensory stimulation compared to the CON group. We suggest that this enhancement might be the result of lowered sensory threshold, although the underlying mechanism has yet to be elucidated.
Objectives: We investigated the effect of dietary supplementation of B-12 on the cortical neural activity of well-nourished young adult rats and tested the hypothesis that B-12 supplementation in healthy rats may reduce sensory evoked neural activity due to enhanced inhibition.
Methods: Female Lister Hooded rats weighing between 190g to 265g (2 to 4 months old) were included in the study. The experimental group was fed with B-12 (Cyanocobalamin) enriched water at a concentration of 1mg/L, and the control (CON) group with tap water for 3 weeks. Animals were then anaesthetised and cortical neural responses to whisker stimulation were recorded in vivo using a multi-channel micro-electrode, from which local field potentials (LFPs) were extracted.
Results: Somatosensory-evoked LFP was enhanced 25.2% in the B-12 group (4.13±0.24mV) compared with the CON group (3.30±0.21mV) (P=0.02). However no significant difference in the frequency spectrum of the spontaneous neural activity was found between the groups, as the unpaired t-statistic at each frequency bin of interest did not pass the cluster-forming threshold of 0.05.
Conclusions: These findings do not provide evidence supporting the hypothesis of decreased neural activity due to B-12 supplementation. As the spontaneous neural activity was unaffected, the increase in somatosensory evoked LFP may be due to enhanced afferent signal reaching the barrel cortex from the whisker pad, indicating that rats supplemented with B-12 may have enhanced sensitivity to sensory stimulation compared to the CON group. We suggest that this enhancement might be the result of lowered sensory threshold, although the underlying mechanism has yet to be elucidated.
| Original language | English |
|---|---|
| Pages (from-to) | 730 -737 |
| Number of pages | 8 |
| Journal | The Journal of Nutrition |
| Volume | 149 |
| Issue number | 5 |
| Early online date | 22 Apr 2019 |
| DOIs | |
| Publication status | Published - 1 May 2019 |
Bibliographical note
© American Society for Nutrition 2019. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.Keywords
- Local field potential
- rat barrel cortex
- vitamin B-12
- dietary supplementation
- sensory threshold
- GABA