Surface-Bound Antibiotic for the Detection of β-Lactamases

TY - JOUR

T1 - Surface-Bound Antibiotic for the Detection of β-Lactamases

AU - Miller, Lisa Margaret

AU - Silver, Callum David

AU - Herman, Reyme

AU - Duhme-Klair, Anne-Kathrin

AU - Thomas, Gavin Hugh

AU - Krauss, Thomas Fraser

AU - Johnson, Steven David

N1 - © 2019 American Chemical Society

PY - 2019/9/11

Y1 - 2019/9/11

N2 - Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests of AMR are necessary. One of the most common and clinically important forms of bacterial resistance is to b-lactam antibiotics (e.g. penicillin). This resistance is often caused by b-lactamases, which hydrolyze b-lactam drugs, rendering them ineffective. Current methods for detecting these enzymes either require time- consuming growth assays or antibiotic mimics such as nitrocefin. Here, we report the development of a surface-bound, clinically- relevant, b-lactam drug that can be used to detect b-lactamases. Furthermore, we demonstrate the use of these functionalized surfaces to selectively detect b-lactamases in complex biological media, such as urine.

AB - Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests of AMR are necessary. One of the most common and clinically important forms of bacterial resistance is to b-lactam antibiotics (e.g. penicillin). This resistance is often caused by b-lactamases, which hydrolyze b-lactam drugs, rendering them ineffective. Current methods for detecting these enzymes either require time- consuming growth assays or antibiotic mimics such as nitrocefin. Here, we report the development of a surface-bound, clinically- relevant, b-lactam drug that can be used to detect b-lactamases. Furthermore, we demonstrate the use of these functionalized surfaces to selectively detect b-lactamases in complex biological media, such as urine.

U2 - 10.1021/acsami.9b05793

DO - 10.1021/acsami.9b05793

M3 - Article

SN - 1944-8244

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

ER -