Synthetic biology approaches to actinomycete strain improvement

Rainer Breitling; Martina Avbelj; Oksana Bilyk; Francesco Del Carratore; Alessandro Filisetti; Erik K.R. Hanko; Marianna Iorio; Rosario Pérez Redondo; Fernando Reyes; Michelle Rudden; Emmanuele Severi; Lucija Slemc; Kamila Schmidt; Dominic R. Whittall; Stefano Donadio; Antonio Rodríguez García; Olga Genilloud; Gregor Kosec; Davide De Lucrezia; Hrvoje Petković; Gavin Thomas; Eriko Takano

doi:10.1093/femsle/fnab060

Synthetic biology approaches to actinomycete strain improvement

Rainer Breitling, Martina Avbelj, Oksana Bilyk, Francesco Del Carratore, Alessandro Filisetti, Erik K.R. Hanko, Marianna Iorio, Rosario Pérez Redondo, Fernando Reyes, Michelle Rudden, Emmanuele Severi, Lucija Slemc, Kamila Schmidt, Dominic R. Whittall, Stefano Donadio, Antonio Rodríguez García, Olga Genilloud, Gregor Kosec, Davide De Lucrezia, Hrvoje PetkovićGavin Thomas, Eriko Takano^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

Abstract

Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains.

Original language	English
Article number	fnab060
Number of pages	9
Journal	FEMS microbiology letters
Volume	368
Issue number	10
DOIs	https://doi.org/10.1093/femsle/fnab060
Publication status	Published - 31 May 2021

Bibliographical note

Funding Information:
This review was funded through the European Union s Horizon 2020 Research and Innovation Programme under grant agreement number 720793 TOPCAPI

Publisher Copyright:
© 2021 The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.

Keywords

Actinobacteria
Actinomycete
Biosynthesis
Biotechnology
Synthetic biology

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10.1093/femsle/fnab060Licence: CC BY

Synthetic biology approaches to actinomycete strain improvement
© The Author(s) 2021.
Final published version, 249 KBLicence: CC BY

Cite this

Breitling, R., Avbelj, M., Bilyk, O., Carratore, F. D., Filisetti, A., Hanko, E. K. R., Iorio, M., Redondo, R. P., Reyes, F., Rudden, M., Severi, E., Slemc, L., Schmidt, K., Whittall, D. R., Donadio, S., García, A. R., Genilloud, O., Kosec, G., De Lucrezia, D., ... Takano, E. (2021). Synthetic biology approaches to actinomycete strain improvement. FEMS microbiology letters, 368(10), Article fnab060. https://doi.org/10.1093/femsle/fnab060

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abstract = "Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains. ",

keywords = "Actinobacteria, Actinomycete, Biosynthesis, Biotechnology, Synthetic biology",

author = "Rainer Breitling and Martina Avbelj and Oksana Bilyk and Carratore, {Francesco Del} and Alessandro Filisetti and Hanko, {Erik K.R.} and Marianna Iorio and Redondo, {Rosario P{\'e}rez} and Fernando Reyes and Michelle Rudden and Emmanuele Severi and Lucija Slemc and Kamila Schmidt and Whittall, {Dominic R.} and Stefano Donadio and Garc{\'i}a, {Antonio Rodr{\'i}guez} and Olga Genilloud and Gregor Kosec and {De Lucrezia}, Davide and Hrvoje Petkovi{\'c} and Gavin Thomas and Eriko Takano",

note = "Funding Information: This review was funded through the European Union s Horizon 2020 Research and Innovation Programme under grant agreement number 720793 TOPCAPI Publisher Copyright: {\textcopyright} 2021 The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.",

year = "2021",

month = may,

day = "31",

doi = "10.1093/femsle/fnab060",

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Breitling, R, Avbelj, M, Bilyk, O, Carratore, FD, Filisetti, A, Hanko, EKR, Iorio, M, Redondo, RP, Reyes, F, Rudden, M , Severi, E, Slemc, L, Schmidt, K, Whittall, DR, Donadio, S, García, AR, Genilloud, O, Kosec, G, De Lucrezia, D, Petković, H, Thomas, G & Takano, E 2021, 'Synthetic biology approaches to actinomycete strain improvement', FEMS microbiology letters, vol. 368, no. 10, fnab060. https://doi.org/10.1093/femsle/fnab060

TY - JOUR

T1 - Synthetic biology approaches to actinomycete strain improvement

AU - Breitling, Rainer

AU - Avbelj, Martina

AU - Bilyk, Oksana

AU - Carratore, Francesco Del

AU - Filisetti, Alessandro

AU - Hanko, Erik K.R.

AU - Iorio, Marianna

AU - Redondo, Rosario Pérez

AU - Reyes, Fernando

AU - Rudden, Michelle

AU - Severi, Emmanuele

AU - Slemc, Lucija

AU - Schmidt, Kamila

AU - Whittall, Dominic R.

AU - Donadio, Stefano

AU - García, Antonio Rodríguez

AU - Genilloud, Olga

AU - Kosec, Gregor

AU - De Lucrezia, Davide

AU - Petković, Hrvoje

AU - Thomas, Gavin

AU - Takano, Eriko

N1 - Funding Information: This review was funded through the European Union s Horizon 2020 Research and Innovation Programme under grant agreement number 720793 TOPCAPI Publisher Copyright: © 2021 The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.

PY - 2021/5/31

Y1 - 2021/5/31

N2 - Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains.

AB - Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains.

KW - Actinobacteria

KW - Actinomycete

KW - Biosynthesis

KW - Biotechnology

KW - Synthetic biology

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U2 - 10.1093/femsle/fnab060

DO - 10.1093/femsle/fnab060

M3 - Review article

C2 - 34057181

AN - SCOPUS:85108304407

SN - 0378-1097

VL - 368

JO - FEMS microbiology letters

JF - FEMS microbiology letters

IS - 10

M1 - fnab060

ER -

Synthetic biology approaches to actinomycete strain improvement

Abstract

Bibliographical note

Keywords

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Thoroughly optimised production chassis for advanced pharmaceutical ingredients

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Synthetic biology approaches to actinomycete strain improvement

Abstract

Bibliographical note

Keywords

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Thoroughly optimised production chassis for advanced pharmaceutical ingredients

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