TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei

Helen P. Price; Meg Stark; Barbara Smith; Deborah F. Smith

doi:10.1016/j.molbiopara.2007.06.009

TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei

Helen P. Price, Meg Stark, Barbara Smith, Deborah F. Smith

Research output: Contribution to journal › Article › peer-review

Abstract

The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contrast, as shown in this study, knockdown of TbARF1 by RNA interference has no effect on fluid-phase endocytosis in the insect stage of the parasite. The protein remains essential for the viability of these procyclic cells but the major effect of TbARF1-depletion is enlargement of the lysosome. Our data indicate that protein trafficking and lysosomal function are differentially regulated by multiple factors, including TbARF1, during progression through the T. brucei lifecyele. (c) 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume	155
Issue number	2
DOIs	https://doi.org/10.1016/j.molbiopara.2007.06.009
Publication status	Published - Oct 2007

Keywords

Trypanosoma brucei
ARF1
endocytosis
lysosome
CLATHRIN-MEDIATED ENDOCYTOSIS
TRAFFICKING
GLYCOPROTEIN
P67

Access to Document

10.1016/j.molbiopara.2007.06.009

Cite this

@article{049301416a9343ff92a7139e1c608b44,

title = "TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei",

abstract = "The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contrast, as shown in this study, knockdown of TbARF1 by RNA interference has no effect on fluid-phase endocytosis in the insect stage of the parasite. The protein remains essential for the viability of these procyclic cells but the major effect of TbARF1-depletion is enlargement of the lysosome. Our data indicate that protein trafficking and lysosomal function are differentially regulated by multiple factors, including TbARF1, during progression through the T. brucei lifecyele. (c) 2007 Elsevier B.V. All rights reserved.",

keywords = "Trypanosoma brucei, ARF1, endocytosis, lysosome, CLATHRIN-MEDIATED ENDOCYTOSIS, TRAFFICKING, GLYCOPROTEIN, P67",

author = "Price, {Helen P.} and Meg Stark and Barbara Smith and Smith, {Deborah F.}",

year = "2007",

month = oct,

doi = "10.1016/j.molbiopara.2007.06.009",

language = "English",

volume = "155",

pages = "123--127",

journal = "MOLECULAR AND BIOCHEMICAL PARASITOLOGY",

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publisher = "Elsevier",

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T1 - TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei

AU - Price, Helen P.

AU - Stark, Meg

AU - Smith, Barbara

AU - Smith, Deborah F.

PY - 2007/10

Y1 - 2007/10

N2 - The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contrast, as shown in this study, knockdown of TbARF1 by RNA interference has no effect on fluid-phase endocytosis in the insect stage of the parasite. The protein remains essential for the viability of these procyclic cells but the major effect of TbARF1-depletion is enlargement of the lysosome. Our data indicate that protein trafficking and lysosomal function are differentially regulated by multiple factors, including TbARF1, during progression through the T. brucei lifecyele. (c) 2007 Elsevier B.V. All rights reserved.

AB - The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contrast, as shown in this study, knockdown of TbARF1 by RNA interference has no effect on fluid-phase endocytosis in the insect stage of the parasite. The protein remains essential for the viability of these procyclic cells but the major effect of TbARF1-depletion is enlargement of the lysosome. Our data indicate that protein trafficking and lysosomal function are differentially regulated by multiple factors, including TbARF1, during progression through the T. brucei lifecyele. (c) 2007 Elsevier B.V. All rights reserved.

KW - Trypanosoma brucei

KW - ARF1

KW - endocytosis

KW - lysosome

KW - CLATHRIN-MEDIATED ENDOCYTOSIS

KW - TRAFFICKING

KW - GLYCOPROTEIN

KW - P67

U2 - 10.1016/j.molbiopara.2007.06.009

DO - 10.1016/j.molbiopara.2007.06.009

M3 - Article

C2 - 17681620

SN - 0166-6851

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SP - 123

EP - 127

JO - MOLECULAR AND BIOCHEMICAL PARASITOLOGY

JF - MOLECULAR AND BIOCHEMICAL PARASITOLOGY

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