Abstract
This pilot study reports the uptake of Tc-99m-transferrin by MCF7 cells and the biodistribution of the complex in mice bearing MCF7 tumours. Human serum holo-transferrin was labelled with Tc-99m using pre-reduction with 2-mercaptoethanol. The uptake of the complex by MCF7 breast tumour cells, which was found to be competitively inhibited by the presence of unlabelled transferrin, reached a plateau within 30 min. Two groups of xenografted mice with small and large tumours, respectively, were injected with the complex, and the uptake was followed for up to 24 h post-administration using a dedicated gamma camera. The mean tumour uptake values, determined after dissection, in the two groups of mice were 6% (small tumours) and 1.7% (large tumours) of the injected dose per gram of tissue, with mean tumour/blood ratios of 2.7 and 1.7, respectively. (C) 2004 Lippincott Williams Wilkins.
Original language | English |
---|---|
Pages (from-to) | 387-391 |
Number of pages | 5 |
Journal | NUCLEAR MEDICINE COMMUNICATIONS |
Volume | 25 |
Issue number | 4 |
DOIs | |
Publication status | Published - Apr 2004 |
Keywords
- Tc-99m-transferrin
- tumour targeting
- biodistribution
- RECEPTOR EXPRESSION
- BINDING
- ANALOG
- CELLS
- ENDOCYTOSIS
- PROTEINS
- CANCER
- TC-99M