By the same authors

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Technical advance: Live-imaging analysis of human dendritic cell migrating behavior under the influence of immune-stimulating reagents in an organotypic model of lung

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Anh Thu Nguyen Hoang
  • Puran Chen
  • Sofia Björnfot
  • Kari Högstrand
  • John G. Lock
  • Alf Grandien
  • Mark Coles
  • Mattias Svensson

Department/unit(s)

Publication details

JournalJournal of leukocyte biology
DateE-pub ahead of print - 14 Jun 2014
DatePublished (current) - Sep 2014
Issue number3
Volume96
Number of pages9
Pages (from-to)481-489
Early online date14/06/14
Original languageEnglish

Abstract

This manuscript describes technical advances allowing manipulation and quantitative analyses of human DC migratory behavior in lung epithelial tissue. DCs are hema-topoietic cells essential for the maintenance of tissue ho-meostasis and the induction of tissue-specific immune responses. Important functions include cytokine production and migration in response to infection for the induction of proper immune responses. To design appropriate strategies to exploit human DC functional properties in lung tissue for the purpose of clinical evaluation, e.g., candidate vaccination and immunotherapy strategies, we have developed a live-imaging assay based on our previously described organotypic model of the human lung. This assay allows provocations and subsequent quantitative investigations of DC functional properties under conditions mimicking morphological and functional features of the in vivo parental tissue. We present protocols to set up and prepare tissue models for 4D (x, y, z, time) fluorescence-imaging analysis that allow spatial and temporal studies of human DCs in live epithelial tissue, followed by flow cytometry analysis of DCs retrieved from digested tissue models. This model system can be useful for elucidating incompletely defined pathways controlling DC functional responses to infection and inflammation in lung epithelial tissue, as well as the efficacy of locally administered candidate interventions. © Society for Leukocyte Biology.

Bibliographical note

© 2014 Society for Leukocyte Biology.

    Research areas

  • 3D-tissue models, Epithelial tissue, Inflammation, Toll-like receptor agonists

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