The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

X. Guo; H. Yang; F. Debelle; G. Yang; J. Denarie; J.P.W. Young; F. Li; X. Zhang; S.L. Turner

The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

X. Guo, H. Yang, F. Debelle, G. Yang, J. Denarie, J.P.W. Young, F. Li, X. Zhang, S.L. Turner

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria.

Original language	English
Pages (from-to)	2988-2995
Number of pages	8
Journal	Applied and Environmental Microbiology
Volume	66
Issue number	7
Publication status	Published - Jul 2000

Bibliographical note

Keywords

NOD FACTORS
NUCLEOTIDE-SEQUENCE
FATTY-ACIDS
MELILOTI
HUAKUII
LEGUMINOSARUM
PROTEINS
IDENTIFICATION
BIOSYNTHESIS
ACTIVATION

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title = "The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity",

abstract = "The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria.",

keywords = "NOD FACTORS, NUCLEOTIDE-SEQUENCE, FATTY-ACIDS, MELILOTI, HUAKUII, LEGUMINOSARUM, PROTEINS, IDENTIFICATION, BIOSYNTHESIS, ACTIVATION",

author = "X. Guo and H. Yang and F. Debelle and G. Yang and J. Denarie and J.P.W. Young and F. Li and X. Zhang and S.L. Turner",

note = "Copyright {\textcopyright} 2000 American Society for Microbiology",

year = "2000",

month = jul,

language = "English",

volume = "66",

pages = "2988--2995",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

AU - Guo, X.

AU - Yang, H.

AU - Debelle, F.

AU - Yang, G.

AU - Denarie, J.

AU - Young, J.P.W.

AU - Li, F.

AU - Zhang, X.

AU - Turner, S.L.

PY - 2000/7

Y1 - 2000/7

N2 - The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria.

AB - The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria.

KW - NOD FACTORS

KW - NUCLEOTIDE-SEQUENCE

KW - FATTY-ACIDS

KW - MELILOTI

KW - HUAKUII

KW - LEGUMINOSARUM

KW - PROTEINS

KW - IDENTIFICATION

KW - BIOSYNTHESIS

KW - ACTIVATION

M3 - Article

SN - 0099-2240

VL - 66

SP - 2988

EP - 2995

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 7

ER -