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The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus

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The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus. / Wells, Rachel; Trick, Martin; Soumpourou, Eleni; Clissold, Leah; Morgan, Colin; Werner, Peter; Gibbard, Carl; Clarke, Matthew; Jennaway, Richard; Bancroft, Ian.

In: Molecular Breeding, Vol. 33, No. 2, 02.2014, p. 349-362.

Research output: Contribution to journalArticle

Harvard

Wells, R, Trick, M, Soumpourou, E, Clissold, L, Morgan, C, Werner, P, Gibbard, C, Clarke, M, Jennaway, R & Bancroft, I 2014, 'The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus', Molecular Breeding, vol. 33, no. 2, pp. 349-362. https://doi.org/10.1007/s11032-013-9954-5

APA

Wells, R., Trick, M., Soumpourou, E., Clissold, L., Morgan, C., Werner, P., ... Bancroft, I. (2014). The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus. Molecular Breeding, 33(2), 349-362. https://doi.org/10.1007/s11032-013-9954-5

Vancouver

Wells R, Trick M, Soumpourou E, Clissold L, Morgan C, Werner P et al. The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus. Molecular Breeding. 2014 Feb;33(2):349-362. https://doi.org/10.1007/s11032-013-9954-5

Author

Wells, Rachel ; Trick, Martin ; Soumpourou, Eleni ; Clissold, Leah ; Morgan, Colin ; Werner, Peter ; Gibbard, Carl ; Clarke, Matthew ; Jennaway, Richard ; Bancroft, Ian. / The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus. In: Molecular Breeding. 2014 ; Vol. 33, No. 2. pp. 349-362.

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@article{262cdb4648d640009a7fc73f999291ad,
title = "The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus",
abstract = "Many important plant species have polyploidy in their recent ancestry, complicating inferences about the genetic basis of trait variation. Although the principal locus controlling the proportion of polyunsaturated fatty acids (PUFAs) in seeds of Arabidopsis thaliana is known (fatty acid desaturase 2; FAD2), commercial cultivars of a related crop, oilseed rape (Brassica napus), with very low PUFA content have yet to be developed. We showed that a cultivar of oilseed rape with lower than usual PUFA content has non-functional alleles at three of the four orthologous FAD2 loci. To explore the genetic basis further, we developed an ethyl methanesulphonate mutagenised population, JBnaCAB_E, and used it to identify lines that also carried mutations in the remaining functional copy. This confirmed the hypothesised basis of variation, resulting in an allelic series of mutant lines showing a spectrum of PUFA contents of seed oil. Several lines had PUFA content of ~6 {\%} and oleic acid content of ~84 {\%}, achieving a long-standing industry objective: very high oleic, very low PUFA rapeseed without the use of genetic modification technology. The population contains a high rate of mutations and represents an important resource for research in B. napus.",
author = "Rachel Wells and Martin Trick and Eleni Soumpourou and Leah Clissold and Colin Morgan and Peter Werner and Carl Gibbard and Matthew Clarke and Richard Jennaway and Ian Bancroft",
year = "2014",
month = "2",
doi = "10.1007/s11032-013-9954-5",
language = "English",
volume = "33",
pages = "349--362",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer Netherlands",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus

AU - Wells, Rachel

AU - Trick, Martin

AU - Soumpourou, Eleni

AU - Clissold, Leah

AU - Morgan, Colin

AU - Werner, Peter

AU - Gibbard, Carl

AU - Clarke, Matthew

AU - Jennaway, Richard

AU - Bancroft, Ian

PY - 2014/2

Y1 - 2014/2

N2 - Many important plant species have polyploidy in their recent ancestry, complicating inferences about the genetic basis of trait variation. Although the principal locus controlling the proportion of polyunsaturated fatty acids (PUFAs) in seeds of Arabidopsis thaliana is known (fatty acid desaturase 2; FAD2), commercial cultivars of a related crop, oilseed rape (Brassica napus), with very low PUFA content have yet to be developed. We showed that a cultivar of oilseed rape with lower than usual PUFA content has non-functional alleles at three of the four orthologous FAD2 loci. To explore the genetic basis further, we developed an ethyl methanesulphonate mutagenised population, JBnaCAB_E, and used it to identify lines that also carried mutations in the remaining functional copy. This confirmed the hypothesised basis of variation, resulting in an allelic series of mutant lines showing a spectrum of PUFA contents of seed oil. Several lines had PUFA content of ~6 % and oleic acid content of ~84 %, achieving a long-standing industry objective: very high oleic, very low PUFA rapeseed without the use of genetic modification technology. The population contains a high rate of mutations and represents an important resource for research in B. napus.

AB - Many important plant species have polyploidy in their recent ancestry, complicating inferences about the genetic basis of trait variation. Although the principal locus controlling the proportion of polyunsaturated fatty acids (PUFAs) in seeds of Arabidopsis thaliana is known (fatty acid desaturase 2; FAD2), commercial cultivars of a related crop, oilseed rape (Brassica napus), with very low PUFA content have yet to be developed. We showed that a cultivar of oilseed rape with lower than usual PUFA content has non-functional alleles at three of the four orthologous FAD2 loci. To explore the genetic basis further, we developed an ethyl methanesulphonate mutagenised population, JBnaCAB_E, and used it to identify lines that also carried mutations in the remaining functional copy. This confirmed the hypothesised basis of variation, resulting in an allelic series of mutant lines showing a spectrum of PUFA contents of seed oil. Several lines had PUFA content of ~6 % and oleic acid content of ~84 %, achieving a long-standing industry objective: very high oleic, very low PUFA rapeseed without the use of genetic modification technology. The population contains a high rate of mutations and represents an important resource for research in B. napus.

UR - http://www.scopus.com/inward/record.url?scp=84893139905&partnerID=8YFLogxK

U2 - 10.1007/s11032-013-9954-5

DO - 10.1007/s11032-013-9954-5

M3 - Article

VL - 33

SP - 349

EP - 362

JO - Molecular Breeding

T2 - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

IS - 2

ER -