The effectiveness of glass beads for plating cell cultures

Michelle Sarah Hawkins; Alidivinas Prusokas; Conrad A Nieduszynski; Renata Retkute

doi:10.1101/241752

The effectiveness of glass beads for plating cell cultures

Michelle Sarah Hawkins, Alidivinas Prusokas, Conrad A Nieduszynski, Renata Retkute

Biology

Research output: Working paper

Abstract

Cell plating and subsequent colony growth is an important laboratory procedure in microbiology. A common protocol for cell culture plating in Petri dishes uses glass beads to spread cells on the surface of the solid growth media. Here we perform an experimental analysis and mathematical modelling of cell plating. We introduce a new class of billiard - shifting billiard, which serves as a conceptual abstraction of the cell plating with glass beads. Numerical analysis of the dynamics of this simple yet efficient billiard, indicates a close relationship between plate movement and quality of colony distribution. Yeast Saccharomyces cerevisiae suspensions at different cell concentrations were used to confirm our theoretical observations.

Original language	English
DOIs	https://doi.org/10.1101/241752
Publication status	Published - 2 Jan 2018

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10.1101/241752

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N2 - Cell plating and subsequent colony growth is an important laboratory procedure in microbiology. A common protocol for cell culture plating in Petri dishes uses glass beads to spread cells on the surface of the solid growth media. Here we perform an experimental analysis and mathematical modelling of cell plating. We introduce a new class of billiard - shifting billiard, which serves as a conceptual abstraction of the cell plating with glass beads. Numerical analysis of the dynamics of this simple yet efficient billiard, indicates a close relationship between plate movement and quality of colony distribution. Yeast Saccharomyces cerevisiae suspensions at different cell concentrations were used to confirm our theoretical observations.

AB - Cell plating and subsequent colony growth is an important laboratory procedure in microbiology. A common protocol for cell culture plating in Petri dishes uses glass beads to spread cells on the surface of the solid growth media. Here we perform an experimental analysis and mathematical modelling of cell plating. We introduce a new class of billiard - shifting billiard, which serves as a conceptual abstraction of the cell plating with glass beads. Numerical analysis of the dynamics of this simple yet efficient billiard, indicates a close relationship between plate movement and quality of colony distribution. Yeast Saccharomyces cerevisiae suspensions at different cell concentrations were used to confirm our theoretical observations.

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