The expression of rice vacuolar TPK channels genes restores potassium uptake in E. coli mutant strain LB2003

TY - JOUR

T1 - The expression of rice vacuolar TPK channels genes restores potassium uptake in E. coli mutant strain LB2003

AU - Isayenkov, S. V.

AU - Maathuis, F. J. M.

PY - 2015/1

Y1 - 2015/1

N2 - The rice genome encodes three different isoforms of two pore potassium channels (TPKs). Recently, the characteristics and features of some rice TPK channels (OsTPKa and OsTPKb) were reported. In order to further evaluate the functional features of these channels, two of the three rice isoforms and one Arabidopsis isoform (AtTPK1) were cloned and expressed in the mutant E. coli strain—LB2003. The E coli LB2003 strain lacks K+ uptake systems and thus is incapable of growing on low external K+ concentrations. The expression of channels in bacteria was confirmed by RT-PCR. Our results show that expression of these channels in the bacterial mutant restored the LB2003 growth on low K+ media. The analysis of potassium uptake exhibited elevated levels of K+ uptake in the three types of TPK transformants. Our data suggest that these plant vacuolar channels can form functional potassium transport systems in bacterial cells.

AB - The rice genome encodes three different isoforms of two pore potassium channels (TPKs). Recently, the characteristics and features of some rice TPK channels (OsTPKa and OsTPKb) were reported. In order to further evaluate the functional features of these channels, two of the three rice isoforms and one Arabidopsis isoform (AtTPK1) were cloned and expressed in the mutant E. coli strain—LB2003. The E coli LB2003 strain lacks K+ uptake systems and thus is incapable of growing on low external K+ concentrations. The expression of channels in bacteria was confirmed by RT-PCR. Our results show that expression of these channels in the bacterial mutant restored the LB2003 growth on low K+ media. The analysis of potassium uptake exhibited elevated levels of K+ uptake in the three types of TPK transformants. Our data suggest that these plant vacuolar channels can form functional potassium transport systems in bacterial cells.

KW - Escherichia coli

KW - Oryza sativa

KW - potassium

KW - TPK

KW - two pore K channels

KW - vacuole

UR - http://www.scopus.com/inward/record.url?scp=84923338863&partnerID=8YFLogxK

U2 - 10.3103/S0095452715010053

DO - 10.3103/S0095452715010053

M3 - Article

AN - SCOPUS:84923338863

SN - 0095-4527

VL - 49

SP - 1

EP - 5

JO - Cytology and Genetics

JF - Cytology and Genetics

IS - 1

ER -