By the same authors

From the same journal

The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases

Research output: Contribution to journalArticle

Author(s)

  • Kristian E H Frandsen
  • Thomas J Simmons
  • Paul Dupree
  • Jens-Christian N. Poulsen
  • Morten Tovborg
  • Katja Salomon Johansen
  • Pernille von Freiesleben
  • Laurence Marmuse
  • Sébastien Fort
  • Sylvain Cottaz
  • Hugues Driguez
  • Bernard Henrissat
  • Nicolas Lenfant
  • Floriana Tuna
  • Amgalanbaatar Baldansuren
  • Leila Lo Leggio

Department/unit(s)

Publication details

JournalNATURE CHEMICAL BIOLOGY
DateAccepted/In press - 12 Jan 2016
DateE-pub ahead of print - 29 Feb 2016
DatePublished (current) - 1 Apr 2016
Issue number4
Volume12
Number of pages6
Pages (from-to)298–303
Early online date29/02/16
Original languageEnglish

Abstract

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.

Bibliographical note

© 2016 Nature America, Inc. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details

    Research areas

  • Amino Acid Sequence, Aspergillus oryzae/enzymology, Binding Sites, Catalytic Domain, Cellulose/metabolism, Chitin/metabolism, Copper/metabolism, Crystallography, X-Ray, Fluorescence Resonance Energy Transfer, Lentinula/enzymology, Mixed Function Oxygenases/chemistry, Models, Molecular, Molecular Sequence Data, Oligosaccharides/chemistry, Oxidation-Reduction, Substrate Specificity

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations