By the same authors

From the same journal

The multifunctional protein AtMFP2 is co-ordinately expressed with other genes of fatty acid beta-oxidation during seed germination in Arabidopsis thaliana (L.) Heynh.

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalBiochemical Society transactions
DatePublished - Feb 2000
Volume28
Number of pages5
Pages (from-to)95-99
Original languageEnglish

Abstract

In germinating oilseeds peroxisomal fatty acid beta-oxidation is responsible for the mobilization of storage lipids. This pathway also occurs in other tissues where it has a variety of additional physiological functions. The central enzymatic steps of peroxisomal beta-oxidation are performed by acyl-CoA oxidase (ACOX), the multifunctional protein (MFP) and 3-ketoacyl-CoA thiolase (thiolase). In order to investigate the function and regulation of beta-oxidation in plants it is first necessary to identify and characterize genes encoding the relevant enzymes in a single model species. Recently we and others have reported on the cloning and characterization of genes encoding four ACOXs and a thiolase from the oilseed Arabidopsis thaliana. Here we identify a gene encoding an Arabidopsis MFP (AtMFP2) that is induced transiently during germination. The pattern of AtMFP2 expression closely reflects changes in the activities of 2-trans-enoyl-CoA hydratase and L-3-hydroxyacyl-CoA dehydrogenase. Similar patterns of expression have previously been reported for ACOX and thiolase genes. We conclude that genes encoding the three main proteins responsible for beta-oxidation are co-ordinately expressed during oilseed germination and may share a common mechanism of regulation.

    Research areas

  • lipid, catabolism, peroxisome, MALATE SYNTHASE GENE, MEDIUM-CHAIN, PLANTS, BIOSYNTHESIS, ENZYMES, THIOESTERASE, PEROXISOMES, ALIGNMENT, THIOLASE, PEPTIDE

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations