The role of zinc in the S100 proteins: Insights from the X-ray structures

TY - JOUR

T1 - The role of zinc in the S100 proteins

T2 - Insights from the X-ray structures

AU - Moroz, Olga V.

AU - Wilson, Keith S.

AU - Bronstein, Igor B.

N1 - Funding Information: This work was supported by European Commission funding through the SPINE2–COMPLEXES project LSHG–CT–2006–031220. We thank Alexei Murzin for the useful discussions of oligomerisation interfaces.

PY - 2011/10

Y1 - 2011/10

N2 - We here aim to summarise the present knowledge on zinc binding by S100 proteins. While the importance of modulation of the function of the S100 family of EF-hand proteins by calcium is well established, a substantial proportion is also regulated by zinc or copper. Indeed regulation by zinc in addition to calcium was suggested almost as soon as the first S100 protein was discovered and has been confirmed for many family members by numerous experiments. For the first, "His-Zn", group, zinc-binding sites composed of three histidines and an aspartic acid were first proposed based on sequence comparisons and later confirmed by structural studies. A second, "Cys-Zn", group lacks such well-defined zinc-binding motifs and for these cysteines were suggested as the main zinc ligands. There is no three-dimensional structure for a Cys-Zn S100 in the presence of zinc. However, analysis of their sequences together with their X-ray structures in the absence of zinc suggests the possibility of two zinc-binding sites: a conserved site with a degree of similarity to those of the His-Zn group and a less-defined site with a Cys interdimer- binding motif. Some S100 protein-mediated events, such as signalling in the extracellular space, where the levels of calcium are already high, are most unlikely to be calcium regulated. Therefore, a broader knowledge of the role of zinc in the functioning of the S100 proteins will add significantly to the understanding how they propagate their signals.

AB - We here aim to summarise the present knowledge on zinc binding by S100 proteins. While the importance of modulation of the function of the S100 family of EF-hand proteins by calcium is well established, a substantial proportion is also regulated by zinc or copper. Indeed regulation by zinc in addition to calcium was suggested almost as soon as the first S100 protein was discovered and has been confirmed for many family members by numerous experiments. For the first, "His-Zn", group, zinc-binding sites composed of three histidines and an aspartic acid were first proposed based on sequence comparisons and later confirmed by structural studies. A second, "Cys-Zn", group lacks such well-defined zinc-binding motifs and for these cysteines were suggested as the main zinc ligands. There is no three-dimensional structure for a Cys-Zn S100 in the presence of zinc. However, analysis of their sequences together with their X-ray structures in the absence of zinc suggests the possibility of two zinc-binding sites: a conserved site with a degree of similarity to those of the His-Zn group and a less-defined site with a Cys interdimer- binding motif. Some S100 protein-mediated events, such as signalling in the extracellular space, where the levels of calcium are already high, are most unlikely to be calcium regulated. Therefore, a broader knowledge of the role of zinc in the functioning of the S100 proteins will add significantly to the understanding how they propagate their signals.

KW - Extracellular signalling

KW - Keywords S100 proteins

KW - X-ray structure

KW - Zinc

KW - Zinc-binding site

UR - http://www.scopus.com/inward/record.url?scp=80855153128&partnerID=8YFLogxK

U2 - 10.1007/s00726-010-0540-4

DO - 10.1007/s00726-010-0540-4

M3 - Review article

C2 - 20306096

AN - SCOPUS:80855153128

SN - 0939-4451

VL - 41

SP - 761

EP - 772

JO - AMINO ACIDS

JF - AMINO ACIDS

IS - 4

ER -