The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5Å resolution

Olga V. Moroz; Michelle Maranta; Tarana Shaghasi; Paul V. Harris; Keith S. Wilson; Gideon J. Davies

doi:10.1107/S2053230X14027307

The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5Å resolution

Olga V. Moroz, Michelle Maranta, Tarana Shaghasi, Paul V. Harris, Keith S. Wilson^*, Gideon J. Davies

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts.

Original language	English
Pages (from-to)	114-120
Number of pages	7
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	71
DOIs	https://doi.org/10.1107/S2053230X14027307
Publication status	Published - 1 Jan 2015

Keywords

Biofuels
Carbohydrate-active enzyme
Cellulase
Thermal stability

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title = "The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5{\AA} resolution",

abstract = "The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5{\AA}, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts.",

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T1 - The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5Å resolution

AU - Moroz, Olga V.

AU - Maranta, Michelle

AU - Shaghasi, Tarana

AU - Harris, Paul V.

AU - Wilson, Keith S.

AU - Davies, Gideon J.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts.

AB - The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts.

KW - Biofuels

KW - Carbohydrate-active enzyme

KW - Cellulase

KW - Thermal stability

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U2 - 10.1107/S2053230X14027307

DO - 10.1107/S2053230X14027307

M3 - Article

SN - 1744-3091

VL - 71

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EP - 120

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

ER -

The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5Å resolution

Abstract

Keywords

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