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The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids

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The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids. / Poppenberger, B; Fujioka, S; Soeno, K; George, G L; Vaistij, F E; Hiranuma, S; Seto, H; Takatsuto, S; Adam, G; Yoshida, S; Bowles, D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 42, 10.2005, p. 15253-15258.

Research output: Contribution to journalArticle

Harvard

Poppenberger, B, Fujioka, S, Soeno, K, George, GL, Vaistij, FE, Hiranuma, S, Seto, H, Takatsuto, S, Adam, G, Yoshida, S & Bowles, D 2005, 'The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 42, pp. 15253-15258. https://doi.org/10.1073/pnas.0504279102

APA

Poppenberger, B., Fujioka, S., Soeno, K., George, G. L., Vaistij, F. E., Hiranuma, S., ... Bowles, D. (2005). The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids. Proceedings of the National Academy of Sciences of the United States of America, 102(42), 15253-15258. https://doi.org/10.1073/pnas.0504279102

Vancouver

Poppenberger B, Fujioka S, Soeno K, George GL, Vaistij FE, Hiranuma S et al. The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids. Proceedings of the National Academy of Sciences of the United States of America. 2005 Oct;102(42):15253-15258. https://doi.org/10.1073/pnas.0504279102

Author

Poppenberger, B ; Fujioka, S ; Soeno, K ; George, G L ; Vaistij, F E ; Hiranuma, S ; Seto, H ; Takatsuto, S ; Adam, G ; Yoshida, S ; Bowles, D. / The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 42. pp. 15253-15258.

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@article{705c2b63c45144e0b2aa7a880105fdbe,
title = "The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids",
abstract = "Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important regulatory role in the activity of steroids in mammals and insects. This study provides an indication that a UGT accepting plant steroids as substrates functions in brassinosteroid (BR) homeostasis. The UGT73C5 of Arabidopsis thaliana catalyses 23-O-glucosylation of the BRs brassinolide (BL) and castasterone. Transgenic plants overexpressing UGT73C5 displayed BR-deficient phenotypes and contained reduced amounts of BRs. The phenotype, which was already apparent in seedlings, could be rescued by application of BR. In feeding experiments with BL, wild-type seedlings converted BL to the 23-O-glucoside; in the transgenic lines silenced in UGT73C5 expression, no 23-O-glucoside was detected, implying that this UGT is the only enzyme that catalyzes BL-23-O-glucosylation in seedlings. Plant lines in which UGT73C5 expression was altered also displayed hypocotyl phenotypes previously described for seedlings in which BR inactivation by hydroxylation was changed. These data support the hypothesis that 23-O-glucosylation of BL is a function of UGT73C5 in planta, and that glucosylation regulates BR activity.",
keywords = "glucosylation, glycosyltransferase, homeostasis, plant, steroid, REGULATED GENE-EXPRESSION, SIGNAL-TRANSDUCTION, BRASSICA-NAPUS, GROWTH, CYTOCHROME-P450, BIOSYNTHESIS, MUTANT, TRANSCRIPTION, INACTIVATION, HORMONES",
author = "B Poppenberger and S Fujioka and K Soeno and George, {G L} and Vaistij, {F E} and S Hiranuma and H Seto and S Takatsuto and G Adam and S Yoshida and D Bowles",
year = "2005",
month = "10",
doi = "10.1073/pnas.0504279102",
language = "English",
volume = "102",
pages = "15253--15258",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "42",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids

AU - Poppenberger, B

AU - Fujioka, S

AU - Soeno, K

AU - George, G L

AU - Vaistij, F E

AU - Hiranuma, S

AU - Seto, H

AU - Takatsuto, S

AU - Adam, G

AU - Yoshida, S

AU - Bowles, D

PY - 2005/10

Y1 - 2005/10

N2 - Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important regulatory role in the activity of steroids in mammals and insects. This study provides an indication that a UGT accepting plant steroids as substrates functions in brassinosteroid (BR) homeostasis. The UGT73C5 of Arabidopsis thaliana catalyses 23-O-glucosylation of the BRs brassinolide (BL) and castasterone. Transgenic plants overexpressing UGT73C5 displayed BR-deficient phenotypes and contained reduced amounts of BRs. The phenotype, which was already apparent in seedlings, could be rescued by application of BR. In feeding experiments with BL, wild-type seedlings converted BL to the 23-O-glucoside; in the transgenic lines silenced in UGT73C5 expression, no 23-O-glucoside was detected, implying that this UGT is the only enzyme that catalyzes BL-23-O-glucosylation in seedlings. Plant lines in which UGT73C5 expression was altered also displayed hypocotyl phenotypes previously described for seedlings in which BR inactivation by hydroxylation was changed. These data support the hypothesis that 23-O-glucosylation of BL is a function of UGT73C5 in planta, and that glucosylation regulates BR activity.

AB - Steroid hormones are essential for development, and the precise control of their homeostasis is a prerequisite for normal growth. UDP-glycosyltransferases (UGTs) are considered to play an important regulatory role in the activity of steroids in mammals and insects. This study provides an indication that a UGT accepting plant steroids as substrates functions in brassinosteroid (BR) homeostasis. The UGT73C5 of Arabidopsis thaliana catalyses 23-O-glucosylation of the BRs brassinolide (BL) and castasterone. Transgenic plants overexpressing UGT73C5 displayed BR-deficient phenotypes and contained reduced amounts of BRs. The phenotype, which was already apparent in seedlings, could be rescued by application of BR. In feeding experiments with BL, wild-type seedlings converted BL to the 23-O-glucoside; in the transgenic lines silenced in UGT73C5 expression, no 23-O-glucoside was detected, implying that this UGT is the only enzyme that catalyzes BL-23-O-glucosylation in seedlings. Plant lines in which UGT73C5 expression was altered also displayed hypocotyl phenotypes previously described for seedlings in which BR inactivation by hydroxylation was changed. These data support the hypothesis that 23-O-glucosylation of BL is a function of UGT73C5 in planta, and that glucosylation regulates BR activity.

KW - glucosylation

KW - glycosyltransferase

KW - homeostasis

KW - plant

KW - steroid

KW - REGULATED GENE-EXPRESSION

KW - SIGNAL-TRANSDUCTION

KW - BRASSICA-NAPUS

KW - GROWTH

KW - CYTOCHROME-P450

KW - BIOSYNTHESIS

KW - MUTANT

KW - TRANSCRIPTION

KW - INACTIVATION

KW - HORMONES

U2 - 10.1073/pnas.0504279102

DO - 10.1073/pnas.0504279102

M3 - Article

VL - 102

SP - 15253

EP - 15258

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 42

ER -