Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors

Benjamin Cressiot, Sandra Joy Muriatu Greive, Mehrnaz Mojtabavi, Alfred Aksel Antson, Meni Wanunu

Research output: Contribution to journalArticlepeer-review

Abstract

Nanopore-based sensors are advancing the sensitivity and selectivity of single-molecule detection in molecular medicine and biotechnology. Current electrical sensing devices are based on either membrane protein pores supported in planar lipid bilayers or solid-state (SS) pores fabricated in thin metallic membranes. While both types of nanosensors have been used in a variety of applications, each has inherent disadvantages that limit its use. Hybrid nanopores, consisting of a protein pore supported within a SS membrane, combine the robust nature of SS membranes with the precise and simple engineering of protein nanopores. We demonstrate here a novel lipid-free hybrid nanopore comprising a natural DNA pore from a thermostable virus, electrokinetically inserted into a larger nanopore supported in a silicon nitride membrane. The hybrid pore is stable and easy to fabricate, and, most importantly, exhibits low peripheral leakage allowing sensing and discrimination among different types of biomolecules.
Original languageEnglish
Article number4652
Pages (from-to)1-7
Number of pages7
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - 7 Nov 2018

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© 2018, The Author(s).

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