TY - JOUR
T1 - TIME for COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
AU - Sanchez-Villarreal, A.
AU - Shin, J.
AU - Bujdoso, N.
AU - Obata, T.
AU - Neumann, U.
AU - Du, S.-X.
AU - Ding, Z.
AU - Davis, A.M.
AU - Shindo, T.
AU - Schmelzer, E.
AU - Sulpice, R.
AU - Nunes-Nesi, A.
AU - Stitt, M.
AU - Fernie, A.R.
AU - Davis, S.J.
PY - 2013/10/1
Y1 - 2013/10/1
N2 - Plants often respond to environmental changes by reprogramming metabolic and stress-associated pathways. Homeostatic integration of signaling is a central requirement for ensuring metabolic stability in living organisms. Under diurnal conditions, properly timed rhythmic metabolism provides fitness benefits to plants. TIME FOR COFFEE (TIC) is a circadian regulator known to be involved in clock resetting at dawn. Here we explored the mechanism of influence of TIC in plant growth and development, as initiated by a microarray analysis. This global profiling showed that a loss of TIC function causes a major reprogramming of gene expression that predicts numerous developmental, metabolic, and stress-related phenotypes. This led us to demonstrate that this mutant exhibits late flowering, a plastochron defect, and diverse anatomical phenotypes. We further observed a starch-excess phenotype and altered soluble carbohydrate levels. tic exhibited hypersensitivity to oxidative stress and abscisic acid, and this was associated with a striking resistance to drought. These phenotypes were connected to an increase in total glutathione levels that correlated with a readjustment of amino acids and polyamine pools. By comparatively analyzing our transcriptomic and metabolomic data, we concluded that TIC is a central element in plant homeostasis that integrates and coordinates developmental, metabolic, and environmental signals.
AB - Plants often respond to environmental changes by reprogramming metabolic and stress-associated pathways. Homeostatic integration of signaling is a central requirement for ensuring metabolic stability in living organisms. Under diurnal conditions, properly timed rhythmic metabolism provides fitness benefits to plants. TIME FOR COFFEE (TIC) is a circadian regulator known to be involved in clock resetting at dawn. Here we explored the mechanism of influence of TIC in plant growth and development, as initiated by a microarray analysis. This global profiling showed that a loss of TIC function causes a major reprogramming of gene expression that predicts numerous developmental, metabolic, and stress-related phenotypes. This led us to demonstrate that this mutant exhibits late flowering, a plastochron defect, and diverse anatomical phenotypes. We further observed a starch-excess phenotype and altered soluble carbohydrate levels. tic exhibited hypersensitivity to oxidative stress and abscisic acid, and this was associated with a striking resistance to drought. These phenotypes were connected to an increase in total glutathione levels that correlated with a readjustment of amino acids and polyamine pools. By comparatively analyzing our transcriptomic and metabolomic data, we concluded that TIC is a central element in plant homeostasis that integrates and coordinates developmental, metabolic, and environmental signals.
UR - http://www.scopus.com/inward/record.url?scp=84885314855&partnerID=8YFLogxK
U2 - 10.1111/tpj.12292
DO - 10.1111/tpj.12292
M3 - Article
AN - SCOPUS:84885314855
SN - 0960-7412
VL - 76
SP - 188
EP - 200
JO - The Plant journal
JF - The Plant journal
IS - 2
ER -