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From the same journal

Time of day modulates low-temperature Ca signals in Arabidopsis

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Author(s)

  • Antony N Dodd
  • Mia Kyed Jakobsen
  • Andrew J Baker
  • Anja Telzerow
  • Sui-Wen Hou
  • Laurent Laplaze
  • Laure Barrot
  • R Scott Poethig
  • Jim Haseloff
  • Alex A R Webb

Department/unit(s)

Publication details

JournalPlant Journal
DatePublished - 2006
Issue number6
Volume48
Number of pages12
Pages (from-to)962-73
Original languageEnglish

Abstract

We tested the hypothesis that the circadian clock modulates Ca(2+)-based signalling pathways, using low-temperature (LT)-induced Ca(2+) signals. We investigated the relationship between diurnal and circadian modulation of LT-induced increases in cytosolic-free calcium ([Ca(2+)](cyt)), and regulation of [Ca(2+)](cyt)-dependent outputs of the LT-signalling network (RD29A transcript abundance and stomatal closure). We measured [Ca(2+)](cyt) non-invasively using aequorin, and targeted aequorin to the guard cell using a guard cell-specific GAL4-green fluorescent protein enhancer trap line. LT caused transient increases in whole plant and guard cell [Ca(2+)](cyt). In guard cells, the LT-induced [Ca(2+)](cyt) elevation preceded stomatal closure. In whole plants, the magnitude of LT-induced [Ca(2+)](cyt) transients, measured from the entire plant or specifically the guard cell, varied with the time of day: LT-induced [Ca(2+)](cyt) transients were significantly higher during the mid-photoperiod than at the beginning or end. Diurnal variation in LT-induced guard cell [Ca(2+)](cyt) increases was not correlated to diurnal variation in LT-induced stomatal closure. There was circadian modulation of LT-induced whole plant [Ca(2+)](cyt) increases, which were correlated to the circadian pattern of RD29A induction. In order to understand the significance of LT-induced [Ca(2+)](cyt) increases, we used a computer simulation to demonstrate that, in guard cells, LT-induced [Ca(2+)](cyt) increases measured from a population of cells are likely to represent the summation of cold-induced single-cell [Ca(2+)](cyt) oscillations.

    Research areas

  • Aequorin, Arabidopsis, Calcium Signaling, Circadian Rhythm, Cold Temperature, Gene Expression Profiling, Green Fluorescent Proteins, Plant Leaves

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