Time-Resolved in situ Raman Spectroscopic Observations of a Biomineralization Model System

Brian Wingender, Emma Tong, John Emery, Laurie Gower, Roland Kröger

Research output: Contribution to journalArticlepeer-review


The mechanisms underlying the formation of nanocrystalline apatite in vertebrate hard tissues (i.e. bones and teeth) remain nebulous. The long-held view of biomineralization via “classical crystallization theory”, or ion-by-ion addition from solution, is challenged by “non-classical” theories in which the pathway proceeds through transient precursor phases [1]. The presence of an amorphous calcium phosphate (ACP) mineral precursor has been observed during the formation of zebrafish fin bones and dental enamel [2, 3]. However, the sample preparation necessary for electron microscopy and ethical considerations inherent to vertebrate samples preclude in situ observations of apatite mineralization.
Original languageEnglish
Pages (from-to)826-827
JournalMicroscopy and Microanalysis
Publication statusPublished - 5 Aug 2019

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