By the same authors

From the same journal

TMEM95 is a sperm membrane protein essential for mammalian fertilization.

Research output: Contribution to journalArticlepeer-review

Full text download(s)


Published copy (DOI)


  • Ismael Lamas-Toranzo
  • Julieta G Hamze
  • Enrica Bianchi
  • Beatriz Fernández-Fuertes
  • Serafín Pérez-Cerezales
  • Ricardo Laguna-Barraza
  • Raúl Fernández-González
  • Pat Lonergan
  • Alfonso Gutiérrez-Adán
  • Gavin J Wright
  • María Jiménez-Movilla
  • Pablo Bermejo-Álvarez


Publication details

DateAccepted/In press - 1 Jun 2020
DatePublished (current) - 2 Jun 2020
Number of pages18
Original languageEnglish


The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable for sperm-egg membrane fusion: the sperm proteins IZUMO1 and SPACA6, and the egg protein JUNO. Here we demonstrate that another sperm protein, TMEM95, is necessary for sperm-egg interaction. TMEM95 ablation in mice caused complete male-specific infertility. Sperm lacking this protein were morphologically normal exhibited normal motility, and could penetrate the zona pellucida and bind to the oolemma. However, once bound to the oolemma, TMEM95-deficient sperm were unable to fuse with the egg membrane or penetrate into the ooplasm, and fertilization could only be achieved by mechanical injection of one sperm into the ooplasm, thereby bypassing membrane fusion. These data demonstrate that TMEM95 is essential for mammalian fertilization. © 2020, Lamas-Toranzo et al.

    Research areas

  • staffpaper

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations