Toward biocompatible nuclear hyperpolarization using signal amplification by reversible exchange: Quantitative in situ spectroscopy and high-field imaging

J.-B. Hövener, N. Schwaderlapp, R. Borowiak, T. Lickert, S.B. Duckett, R.E. Mewis, R.W. Adams, M.J. Burns, L.A.R. Highton, G.G.R. Green, A. Olaru, J. Hennig, D. Von Elverfeldt

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Signal amplification by reversible exchange (SABRE) of a substrate and parahydrogen at a catalytic center promises to overcome the inherent insensitivity of magnetic resonance. In order to apply the new approach to biomedical applications, there is a need to develop experimental equipment, in situ quantification methods, and a biocompatible solvent. We present results detailing a low-field SABRE polarizer which provides well-controlled experimental conditions, defined spins manipulations, and which allows in situ detection of thermally polarized and hyperpolarized samples. We introduce a method for absolute quantification of hyperpolarization yield in situ by means of a thermally polarized reference. A maximum signal-to-noise ratio of ∼10 for 148 μmol of substance, a signal enhancement of 10 with respect to polarization transfer field of SABRE, or an absolute H-polarization level of ≈10 is achieved. In an important step toward biomedical application, we demonstrate H in situ NMR as well as H and C high-field MRI using hyperpolarized pyridine (d) and C nicotinamide in pure and 11% ethanol in aqueous solution. Further increase of hyperpolarization yield, implications of in situ detection, and in vivo application are discussed.
Original languageEnglish
Pages (from-to)1767-1774
Number of pages8
JournalAnalytical Chemistry
Issue number3
Early online date24 Jan 2014
Publication statusPublished - 4 Feb 2014

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