Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder

C Booth; P Harnden; P J Selby; J Southgate

doi:10.1002/path.1214

Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder

C Booth, P Harnden, P J Selby, J Southgate

Jack Birch Unit

Research output: Contribution to journal › Article › peer-review

Abstract

Tenascin-C (TN-C) is an extracellular matrix glycoprotein expressed along epithelial/stromal boundaries during tissue remodelling events, such as those that occur during morphogenesis, wound healing, and tumour invasion. Using clinical specimens and a range of in vitro models that simulate homeostasis, wound healing, and malignant progression, this study sought to establish the patterns of TN-C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta-1 (TGFbeta-1), interleukin-4 (IL-4), basic fibroblast growth factor (bFGF), tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in the induction of TN-C expression by bladder uro-epithelial cells. The findings indicate that normal urothelial cells may express TN-C, with both TGFbeta-1 and IL-4 able to induce expression. TN-C was not expressed in neoplastic urothelium, although both TN-C and TGFbeta-1 may be involved in tissue remodelling during papillary tumour formation and invasion. Furthermore, the urothelium of high-grade papillary tumours and carcinoma in situ specimens exhibited little TGFbeta-1 immunoreactivity, compared with the urothelium of low-grade tumours and normal specimens, suggesting an association between TGFbeta-1 expression and urothelial differentiation. A tumour invasion model, in which established bladder cancer cell lines were seeded onto a normal bladder stroma, corroborated the evidence from the clinical specimens and demonstrated that TN-C was strongly expressed around foci of stromal invasion. Thus, TN-C immunoreactivity may provide an additional tool in the assessment of early stromal invasion in bladder cancer. Copyright (C) 2002 John Wiley Sons, Ltd.

Original language	English
Pages (from-to)	359-368
Number of pages	10
Journal	Journal of Pathology
Volume	198
Issue number	3
DOIs	https://doi.org/10.1002/path.1214
Publication status	Published - Nov 2002

Keywords

bladder cancer
transitional cell carcinoma
tenascin
transforming growth factor beta 1
urothelium
TRANSITIONAL-CELL-CARCINOMA
GROWTH-FACTOR-BETA
EPITHELIAL-MESENCHYMAL INTERACTIONS
HUMAN UROTHELIAL CELLS
IN-VITRO
ENDOTHELIAL-CELLS
BASEMENT-MEMBRANE
TUMOR PROGRESSION
EXPRESSION
TISSUE

Access to Document

10.1002/path.1214

Cite this

@article{4f2bd5cca9174fedaf156585ebb7a617,

title = "Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder",

abstract = "Tenascin-C (TN-C) is an extracellular matrix glycoprotein expressed along epithelial/stromal boundaries during tissue remodelling events, such as those that occur during morphogenesis, wound healing, and tumour invasion. Using clinical specimens and a range of in vitro models that simulate homeostasis, wound healing, and malignant progression, this study sought to establish the patterns of TN-C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta-1 (TGFbeta-1), interleukin-4 (IL-4), basic fibroblast growth factor (bFGF), tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in the induction of TN-C expression by bladder uro-epithelial cells. The findings indicate that normal urothelial cells may express TN-C, with both TGFbeta-1 and IL-4 able to induce expression. TN-C was not expressed in neoplastic urothelium, although both TN-C and TGFbeta-1 may be involved in tissue remodelling during papillary tumour formation and invasion. Furthermore, the urothelium of high-grade papillary tumours and carcinoma in situ specimens exhibited little TGFbeta-1 immunoreactivity, compared with the urothelium of low-grade tumours and normal specimens, suggesting an association between TGFbeta-1 expression and urothelial differentiation. A tumour invasion model, in which established bladder cancer cell lines were seeded onto a normal bladder stroma, corroborated the evidence from the clinical specimens and demonstrated that TN-C was strongly expressed around foci of stromal invasion. Thus, TN-C immunoreactivity may provide an additional tool in the assessment of early stromal invasion in bladder cancer. Copyright (C) 2002 John Wiley Sons, Ltd.",

keywords = "bladder cancer, transitional cell carcinoma, tenascin, transforming growth factor beta 1, urothelium, TRANSITIONAL-CELL-CARCINOMA, GROWTH-FACTOR-BETA, EPITHELIAL-MESENCHYMAL INTERACTIONS, HUMAN UROTHELIAL CELLS, IN-VITRO, ENDOTHELIAL-CELLS, BASEMENT-MEMBRANE, TUMOR PROGRESSION, EXPRESSION, TISSUE",

author = "C Booth and P Harnden and Selby, {P J} and J Southgate",

year = "2002",

month = nov,

doi = "10.1002/path.1214",

language = "English",

volume = "198",

pages = "359--368",

journal = "Journal of Pathology",

issn = "0022-3417",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder

AU - Booth, C

AU - Harnden, P

AU - Selby, P J

AU - Southgate, J

PY - 2002/11

Y1 - 2002/11

N2 - Tenascin-C (TN-C) is an extracellular matrix glycoprotein expressed along epithelial/stromal boundaries during tissue remodelling events, such as those that occur during morphogenesis, wound healing, and tumour invasion. Using clinical specimens and a range of in vitro models that simulate homeostasis, wound healing, and malignant progression, this study sought to establish the patterns of TN-C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta-1 (TGFbeta-1), interleukin-4 (IL-4), basic fibroblast growth factor (bFGF), tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in the induction of TN-C expression by bladder uro-epithelial cells. The findings indicate that normal urothelial cells may express TN-C, with both TGFbeta-1 and IL-4 able to induce expression. TN-C was not expressed in neoplastic urothelium, although both TN-C and TGFbeta-1 may be involved in tissue remodelling during papillary tumour formation and invasion. Furthermore, the urothelium of high-grade papillary tumours and carcinoma in situ specimens exhibited little TGFbeta-1 immunoreactivity, compared with the urothelium of low-grade tumours and normal specimens, suggesting an association between TGFbeta-1 expression and urothelial differentiation. A tumour invasion model, in which established bladder cancer cell lines were seeded onto a normal bladder stroma, corroborated the evidence from the clinical specimens and demonstrated that TN-C was strongly expressed around foci of stromal invasion. Thus, TN-C immunoreactivity may provide an additional tool in the assessment of early stromal invasion in bladder cancer. Copyright (C) 2002 John Wiley Sons, Ltd.

AB - Tenascin-C (TN-C) is an extracellular matrix glycoprotein expressed along epithelial/stromal boundaries during tissue remodelling events, such as those that occur during morphogenesis, wound healing, and tumour invasion. Using clinical specimens and a range of in vitro models that simulate homeostasis, wound healing, and malignant progression, this study sought to establish the patterns of TN-C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta-1 (TGFbeta-1), interleukin-4 (IL-4), basic fibroblast growth factor (bFGF), tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in the induction of TN-C expression by bladder uro-epithelial cells. The findings indicate that normal urothelial cells may express TN-C, with both TGFbeta-1 and IL-4 able to induce expression. TN-C was not expressed in neoplastic urothelium, although both TN-C and TGFbeta-1 may be involved in tissue remodelling during papillary tumour formation and invasion. Furthermore, the urothelium of high-grade papillary tumours and carcinoma in situ specimens exhibited little TGFbeta-1 immunoreactivity, compared with the urothelium of low-grade tumours and normal specimens, suggesting an association between TGFbeta-1 expression and urothelial differentiation. A tumour invasion model, in which established bladder cancer cell lines were seeded onto a normal bladder stroma, corroborated the evidence from the clinical specimens and demonstrated that TN-C was strongly expressed around foci of stromal invasion. Thus, TN-C immunoreactivity may provide an additional tool in the assessment of early stromal invasion in bladder cancer. Copyright (C) 2002 John Wiley Sons, Ltd.

KW - bladder cancer

KW - transitional cell carcinoma

KW - tenascin

KW - transforming growth factor beta 1

KW - urothelium

KW - TRANSITIONAL-CELL-CARCINOMA

KW - GROWTH-FACTOR-BETA

KW - EPITHELIAL-MESENCHYMAL INTERACTIONS

KW - HUMAN UROTHELIAL CELLS

KW - IN-VITRO

KW - ENDOTHELIAL-CELLS

KW - BASEMENT-MEMBRANE

KW - TUMOR PROGRESSION

KW - EXPRESSION

KW - TISSUE

U2 - 10.1002/path.1214

DO - 10.1002/path.1214

M3 - Article

SN - 0022-3417

VL - 198

SP - 359

EP - 368

JO - Journal of Pathology

JF - Journal of Pathology

IS - 3

ER -