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Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder

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Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder. / Booth, C; Harnden, P; Selby, P J; Southgate, J.

In: Journal of Pathology, Vol. 198, No. 3, 11.2002, p. 359-368.

Research output: Contribution to journalArticle

Harvard

Booth, C, Harnden, P, Selby, PJ & Southgate, J 2002, 'Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder', Journal of Pathology, vol. 198, no. 3, pp. 359-368. https://doi.org/10.1002/path.1214

APA

Booth, C., Harnden, P., Selby, P. J., & Southgate, J. (2002). Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder. Journal of Pathology, 198(3), 359-368. https://doi.org/10.1002/path.1214

Vancouver

Booth C, Harnden P, Selby PJ, Southgate J. Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder. Journal of Pathology. 2002 Nov;198(3):359-368. https://doi.org/10.1002/path.1214

Author

Booth, C ; Harnden, P ; Selby, P J ; Southgate, J. / Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder. In: Journal of Pathology. 2002 ; Vol. 198, No. 3. pp. 359-368.

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@article{4f2bd5cca9174fedaf156585ebb7a617,
title = "Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder",
abstract = "Tenascin-C (TN-C) is an extracellular matrix glycoprotein expressed along epithelial/stromal boundaries during tissue remodelling events, such as those that occur during morphogenesis, wound healing, and tumour invasion. Using clinical specimens and a range of in vitro models that simulate homeostasis, wound healing, and malignant progression, this study sought to establish the patterns of TN-C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta-1 (TGFbeta-1), interleukin-4 (IL-4), basic fibroblast growth factor (bFGF), tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in the induction of TN-C expression by bladder uro-epithelial cells. The findings indicate that normal urothelial cells may express TN-C, with both TGFbeta-1 and IL-4 able to induce expression. TN-C was not expressed in neoplastic urothelium, although both TN-C and TGFbeta-1 may be involved in tissue remodelling during papillary tumour formation and invasion. Furthermore, the urothelium of high-grade papillary tumours and carcinoma in situ specimens exhibited little TGFbeta-1 immunoreactivity, compared with the urothelium of low-grade tumours and normal specimens, suggesting an association between TGFbeta-1 expression and urothelial differentiation. A tumour invasion model, in which established bladder cancer cell lines were seeded onto a normal bladder stroma, corroborated the evidence from the clinical specimens and demonstrated that TN-C was strongly expressed around foci of stromal invasion. Thus, TN-C immunoreactivity may provide an additional tool in the assessment of early stromal invasion in bladder cancer. Copyright (C) 2002 John Wiley Sons, Ltd.",
keywords = "bladder cancer, transitional cell carcinoma, tenascin, transforming growth factor beta 1, urothelium, TRANSITIONAL-CELL-CARCINOMA, GROWTH-FACTOR-BETA, EPITHELIAL-MESENCHYMAL INTERACTIONS, HUMAN UROTHELIAL CELLS, IN-VITRO, ENDOTHELIAL-CELLS, BASEMENT-MEMBRANE, TUMOR PROGRESSION, EXPRESSION, TISSUE",
author = "C Booth and P Harnden and Selby, {P J} and J Southgate",
year = "2002",
month = "11",
doi = "10.1002/path.1214",
language = "English",
volume = "198",
pages = "359--368",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Towards defining roles and relationships for tenascin-C and TGF beta-1 in the normal and neoplastic urinary bladder

AU - Booth, C

AU - Harnden, P

AU - Selby, P J

AU - Southgate, J

PY - 2002/11

Y1 - 2002/11

N2 - Tenascin-C (TN-C) is an extracellular matrix glycoprotein expressed along epithelial/stromal boundaries during tissue remodelling events, such as those that occur during morphogenesis, wound healing, and tumour invasion. Using clinical specimens and a range of in vitro models that simulate homeostasis, wound healing, and malignant progression, this study sought to establish the patterns of TN-C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta-1 (TGFbeta-1), interleukin-4 (IL-4), basic fibroblast growth factor (bFGF), tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in the induction of TN-C expression by bladder uro-epithelial cells. The findings indicate that normal urothelial cells may express TN-C, with both TGFbeta-1 and IL-4 able to induce expression. TN-C was not expressed in neoplastic urothelium, although both TN-C and TGFbeta-1 may be involved in tissue remodelling during papillary tumour formation and invasion. Furthermore, the urothelium of high-grade papillary tumours and carcinoma in situ specimens exhibited little TGFbeta-1 immunoreactivity, compared with the urothelium of low-grade tumours and normal specimens, suggesting an association between TGFbeta-1 expression and urothelial differentiation. A tumour invasion model, in which established bladder cancer cell lines were seeded onto a normal bladder stroma, corroborated the evidence from the clinical specimens and demonstrated that TN-C was strongly expressed around foci of stromal invasion. Thus, TN-C immunoreactivity may provide an additional tool in the assessment of early stromal invasion in bladder cancer. Copyright (C) 2002 John Wiley Sons, Ltd.

AB - Tenascin-C (TN-C) is an extracellular matrix glycoprotein expressed along epithelial/stromal boundaries during tissue remodelling events, such as those that occur during morphogenesis, wound healing, and tumour invasion. Using clinical specimens and a range of in vitro models that simulate homeostasis, wound healing, and malignant progression, this study sought to establish the patterns of TN-C expression in normal and neoplastic bladder and to determine the role of exogenous transforming growth factor beta-1 (TGFbeta-1), interleukin-4 (IL-4), basic fibroblast growth factor (bFGF), tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) in the induction of TN-C expression by bladder uro-epithelial cells. The findings indicate that normal urothelial cells may express TN-C, with both TGFbeta-1 and IL-4 able to induce expression. TN-C was not expressed in neoplastic urothelium, although both TN-C and TGFbeta-1 may be involved in tissue remodelling during papillary tumour formation and invasion. Furthermore, the urothelium of high-grade papillary tumours and carcinoma in situ specimens exhibited little TGFbeta-1 immunoreactivity, compared with the urothelium of low-grade tumours and normal specimens, suggesting an association between TGFbeta-1 expression and urothelial differentiation. A tumour invasion model, in which established bladder cancer cell lines were seeded onto a normal bladder stroma, corroborated the evidence from the clinical specimens and demonstrated that TN-C was strongly expressed around foci of stromal invasion. Thus, TN-C immunoreactivity may provide an additional tool in the assessment of early stromal invasion in bladder cancer. Copyright (C) 2002 John Wiley Sons, Ltd.

KW - bladder cancer

KW - transitional cell carcinoma

KW - tenascin

KW - transforming growth factor beta 1

KW - urothelium

KW - TRANSITIONAL-CELL-CARCINOMA

KW - GROWTH-FACTOR-BETA

KW - EPITHELIAL-MESENCHYMAL INTERACTIONS

KW - HUMAN UROTHELIAL CELLS

KW - IN-VITRO

KW - ENDOTHELIAL-CELLS

KW - BASEMENT-MEMBRANE

KW - TUMOR PROGRESSION

KW - EXPRESSION

KW - TISSUE

U2 - 10.1002/path.1214

DO - 10.1002/path.1214

M3 - Article

VL - 198

SP - 359

EP - 368

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 3

ER -