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Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells

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Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells. / Wollman, Adam; Hedlund, Erik Goran; Shashkova, Sviatlana; Leake, Mark Christian.

In: Methods, 26.06.2019.

Research output: Contribution to journalArticlepeer-review

Harvard

Wollman, A, Hedlund, EG, Shashkova, S & Leake, MC 2019, 'Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells', Methods. https://doi.org/10.1016/j.ymeth.2019.06.021

APA

Wollman, A., Hedlund, E. G., Shashkova, S., & Leake, M. C. (2019). Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells. Methods. https://doi.org/10.1016/j.ymeth.2019.06.021

Vancouver

Wollman A, Hedlund EG, Shashkova S, Leake MC. Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells. Methods. 2019 Jun 26. https://doi.org/10.1016/j.ymeth.2019.06.021

Author

Wollman, Adam ; Hedlund, Erik Goran ; Shashkova, Sviatlana ; Leake, Mark Christian. / Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells. In: Methods. 2019.

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@article{4fb293c4dea5412d9c9afa5c751743fc,
title = "Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells",
abstract = "How genomic DNA is organized in the nucleus is a long-standing question. We describe a single-molecule bioimaging method utilizing super-localization precision coupled to fully quantitative image analysis tools, towards determining snapshots of parts of the 3D genome architecture of model eukaryote budding yeast Saccharomyces cerevisiae with exceptional millisecond time resolution. We employ astigmatism imaging to enable robust extraction of 3D position data on genomically encoded fluorescent protein reporters that bind to DNA. Our relatively straightforward method enables snippets of 3D architectures of likely single genome conformations to be resolved captured via DNA-sequence specific binding proteins in single functional living cells.",
keywords = "Gene regulation, Single-molecule, Super-resolution, Transcription, Transcription factors, Yeast genome",
author = "Adam Wollman and Hedlund, {Erik Goran} and Sviatlana Shashkova and Leake, {Mark Christian}",
year = "2019",
month = jun,
day = "26",
doi = "10.1016/j.ymeth.2019.06.021",
language = "English",
journal = "Methods",
issn = "1046-2023",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells

AU - Wollman, Adam

AU - Hedlund, Erik Goran

AU - Shashkova, Sviatlana

AU - Leake, Mark Christian

PY - 2019/6/26

Y1 - 2019/6/26

N2 - How genomic DNA is organized in the nucleus is a long-standing question. We describe a single-molecule bioimaging method utilizing super-localization precision coupled to fully quantitative image analysis tools, towards determining snapshots of parts of the 3D genome architecture of model eukaryote budding yeast Saccharomyces cerevisiae with exceptional millisecond time resolution. We employ astigmatism imaging to enable robust extraction of 3D position data on genomically encoded fluorescent protein reporters that bind to DNA. Our relatively straightforward method enables snippets of 3D architectures of likely single genome conformations to be resolved captured via DNA-sequence specific binding proteins in single functional living cells.

AB - How genomic DNA is organized in the nucleus is a long-standing question. We describe a single-molecule bioimaging method utilizing super-localization precision coupled to fully quantitative image analysis tools, towards determining snapshots of parts of the 3D genome architecture of model eukaryote budding yeast Saccharomyces cerevisiae with exceptional millisecond time resolution. We employ astigmatism imaging to enable robust extraction of 3D position data on genomically encoded fluorescent protein reporters that bind to DNA. Our relatively straightforward method enables snippets of 3D architectures of likely single genome conformations to be resolved captured via DNA-sequence specific binding proteins in single functional living cells.

KW - Gene regulation

KW - Single-molecule

KW - Super-resolution

KW - Transcription

KW - Transcription factors

KW - Yeast genome

UR - http://www.scopus.com/inward/record.url?scp=85068189701&partnerID=8YFLogxK

U2 - 10.1016/j.ymeth.2019.06.021

DO - 10.1016/j.ymeth.2019.06.021

M3 - Article

JO - Methods

JF - Methods

SN - 1046-2023

ER -