Toxicity of tryptophan manganese(I) carbonyl (Trypto-CORM), against Neisseria gonorrhoeae

Jonathan S. Ward; Rebecca Morgan; Jason M. Lynam; Ian J S Fairlamb; James W B Moir

doi:10.1039/C6MD00603E

Toxicity of tryptophan manganese(I) carbonyl (Trypto-CORM), against Neisseria gonorrhoeae

Jonathan S. Ward, Rebecca Morgan, Jason M. Lynam, Ian J S Fairlamb, James W B Moir^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The potential for carbon monoxide-releasing molecules (CO-RMs) as antimicrobials represents an exciting prospective in the fight against antibiotic resistance. Trypto-CORM, a tryptophan-containing manganese(i) carbonyl, is toxic against E. coli following photo-activation. Here, we demonstrate that Trypto-CORM is toxic against Neisseria gonorrhoeae in the absence of photoactivation. Trypto-CORM toxicity was reversed by the high CO affinity globin leg-haemoglobin (Leg-Hb), indicating that the toxicity is due to CO release. Release of CO from Trypto-CORM in the dark was also detected with Leg-Hb (but not myoglobin) in vitro. N. gonorrhoeae is more sensitive to CO-based toxicity than other model bacterial pathogens, and may serve as a viable candidate for antimicrobial therapy using CO-RMs.

Original language	English
Pages (from-to)	346-352
Number of pages	7
Journal	MedChemComm
Volume	8
Issue number	2
Early online date	6 Dec 2016
DOIs	https://doi.org/10.1039/C6MD00603E
Publication status	Published - 2017

Bibliographical note

© The Royal Society of Chemistry 2016. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

Access to Document

10.1039/C6MD00603E

Moir AAM Ward et al.docx -revised
© The Royal Society of Chemistry 2016. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.
Accepted author manuscript, 685 KB

Cite this

@article{7254b1360d1a4a2a92cbb8dced2e7162,

title = "Toxicity of tryptophan manganese(I) carbonyl (Trypto-CORM), against Neisseria gonorrhoeae",

abstract = "The potential for carbon monoxide-releasing molecules (CO-RMs) as antimicrobials represents an exciting prospective in the fight against antibiotic resistance. Trypto-CORM, a tryptophan-containing manganese(i) carbonyl, is toxic against E. coli following photo-activation. Here, we demonstrate that Trypto-CORM is toxic against Neisseria gonorrhoeae in the absence of photoactivation. Trypto-CORM toxicity was reversed by the high CO affinity globin leg-haemoglobin (Leg-Hb), indicating that the toxicity is due to CO release. Release of CO from Trypto-CORM in the dark was also detected with Leg-Hb (but not myoglobin) in vitro. N. gonorrhoeae is more sensitive to CO-based toxicity than other model bacterial pathogens, and may serve as a viable candidate for antimicrobial therapy using CO-RMs.",

author = "Ward, {Jonathan S.} and Rebecca Morgan and Lynam, {Jason M.} and Fairlamb, {Ian J S} and Moir, {James W B}",

note = " {\textcopyright} The Royal Society of Chemistry 2016. This is an author-produced version of the published paper. Uploaded in accordance with the publisher{\textquoteright}s self-archiving policy. Further copying may not be permitted; contact the publisher for details. ",

year = "2017",

doi = "10.1039/C6MD00603E",

language = "English",

volume = "8",

pages = "346--352",

journal = "MedChemComm",

issn = "2040-2503",

publisher = "The Royal Society of Chemistry",

number = "2",

}

TY - JOUR

T1 - Toxicity of tryptophan manganese(I) carbonyl (Trypto-CORM), against Neisseria gonorrhoeae

AU - Ward, Jonathan S.

AU - Morgan, Rebecca

AU - Lynam, Jason M.

AU - Fairlamb, Ian J S

AU - Moir, James W B

N1 - © The Royal Society of Chemistry 2016. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details.

PY - 2017

Y1 - 2017

N2 - The potential for carbon monoxide-releasing molecules (CO-RMs) as antimicrobials represents an exciting prospective in the fight against antibiotic resistance. Trypto-CORM, a tryptophan-containing manganese(i) carbonyl, is toxic against E. coli following photo-activation. Here, we demonstrate that Trypto-CORM is toxic against Neisseria gonorrhoeae in the absence of photoactivation. Trypto-CORM toxicity was reversed by the high CO affinity globin leg-haemoglobin (Leg-Hb), indicating that the toxicity is due to CO release. Release of CO from Trypto-CORM in the dark was also detected with Leg-Hb (but not myoglobin) in vitro. N. gonorrhoeae is more sensitive to CO-based toxicity than other model bacterial pathogens, and may serve as a viable candidate for antimicrobial therapy using CO-RMs.

AB - The potential for carbon monoxide-releasing molecules (CO-RMs) as antimicrobials represents an exciting prospective in the fight against antibiotic resistance. Trypto-CORM, a tryptophan-containing manganese(i) carbonyl, is toxic against E. coli following photo-activation. Here, we demonstrate that Trypto-CORM is toxic against Neisseria gonorrhoeae in the absence of photoactivation. Trypto-CORM toxicity was reversed by the high CO affinity globin leg-haemoglobin (Leg-Hb), indicating that the toxicity is due to CO release. Release of CO from Trypto-CORM in the dark was also detected with Leg-Hb (but not myoglobin) in vitro. N. gonorrhoeae is more sensitive to CO-based toxicity than other model bacterial pathogens, and may serve as a viable candidate for antimicrobial therapy using CO-RMs.

UR - http://www.scopus.com/inward/record.url?scp=85013831078&partnerID=8YFLogxK

U2 - 10.1039/C6MD00603E

DO - 10.1039/C6MD00603E

M3 - Article

AN - SCOPUS:85013831078

SN - 2040-2503

VL - 8

SP - 346

EP - 352

JO - MedChemComm

JF - MedChemComm

IS - 2

ER -

Toxicity of tryptophan manganese(I) carbonyl (Trypto-CORM), against Neisseria gonorrhoeae

Abstract

Bibliographical note

Access to Document

Other files and links

Cite this