Transcriptional dynamics driving MAMP-triggered immunity and pathogen effector-mediated immunosuppression in Arabidopsis leaves following infection with Pseudomonas syringae pv tomato DC3000

Laura A. Lewis, Krzysztof Polanski, Marta de Torres-Zabala, Siddharth Jayaraman, Laura Bowden, Jonathan Moore, Christopher A. Penfold, Dafyd J. Jenkins, Claire Hill, Laura Baxter, Satish Kulasekaran, William Truman, George Littlejohn, Justyna Prusinska, Andrew Mead, Jens Steinbrenner, Richard Hickman, David Rand, David L. Wild, Sascha OttVicky Buchanan-Wollaston, Nick Smirnoff, Jim Beynon, Katherine Denby, Murray Grant*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Transcriptional reprogramming is integral to effective plant defense. Pathogen effectors act transcriptionally and posttranscriptionally to suppress defense responses. A major challenge to understanding disease and defense responses is discriminating between transcriptional reprogramming associated with microbial-associated molecular pattern (MAMP)-triggered immunity (MTI) and that orchestrated by effectors. A high-resolution time course of genome-wide expression changes following challenge with Pseudomonas syringae pv tomato DC3000 and the nonpathogenic mutant strain DC3000hrpA allowed us to establish causal links between the activities of pathogen effectors and suppression of MTI and infer with high confidence a range of processes specifically targeted by effectors. Analysis of this information-rich data set with a range of computational tools provided insights into the earliest transcriptional events triggered by effector delivery, regulatory mechanisms recruited, and biological processes targeted. We show that the majority of genes contributing to disease or defense are induced within 6 h postinfection, significantly before pathogen multiplication. Suppression of chloroplast-associated genes is a rapid MAMP-triggered defense response, and suppression of genes involved in chromatin assembly and induction of ubiquitin-related genes coincide with pathogen-induced abscisic acid accumulation. Specific combinations of promoter motifs are engaged in fine-tuning the MTI response and active transcriptional suppression at specific promoter configurations by P. syringae.

Original languageEnglish
Pages (from-to)3038-3064
Number of pages27
JournalThe Plant Cell
Issue number11
Publication statusPublished - 13 Nov 2015

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