Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication

Emma L Hesketh, John RP Knight, Rosemary HC Wilson, James PJ Chong, Dawn Coverley

Research output: Contribution to journalArticlepeer-review


The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as cells pass through late G1 and early S phase, identifying a brief window when MCM2 becomes transiently attached to the nuclear-matrix. The data distinguish 3 states that correspond to loose association with chromatin prior to DNA replication, transient highly stable binding to the nuclear-matrix coincident with initiation, and a post-initiation phase when MCM2 remains tightly associated with chromatin but not the nuclear-matrix. The data suggests that functional MCM complex loading takes place at the nuclear-matrix.

Original languageEnglish
Pages (from-to)333-41
Number of pages9
JournalCell cycle
Issue number3
Publication statusPublished - 6 Feb 2015


  • Cell cycle
  • DNA replication
  • Inititation
  • Minichromosome maintenance complex
  • MCM2-7
  • Nuclear matrix
  • NM
  • MCM
  • Minichromosome Maintenance

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