Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication

Emma L Hesketh; John RP Knight; Rosemary HC Wilson; James PJ Chong; Dawn Coverley

doi:10.4161/15384101.2014.980647

Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication

Emma L Hesketh, John RP Knight, Rosemary HC Wilson, James PJ Chong, Dawn Coverley

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as cells pass through late G1 and early S phase, identifying a brief window when MCM2 becomes transiently attached to the nuclear-matrix. The data distinguish 3 states that correspond to loose association with chromatin prior to DNA replication, transient highly stable binding to the nuclear-matrix coincident with initiation, and a post-initiation phase when MCM2 remains tightly associated with chromatin but not the nuclear-matrix. The data suggests that functional MCM complex loading takes place at the nuclear-matrix.

Original language	English
Pages (from-to)	333-41
Number of pages	9
Journal	Cell cycle
Volume	14
Issue number	3
DOIs	https://doi.org/10.4161/15384101.2014.980647
Publication status	Published - 6 Feb 2015

Keywords

Cell cycle
DNA replication
Inititation
Minichromosome maintenance complex
MCM2-7
Nuclear matrix
NM
MCM
Minichromosome Maintenance

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10.4161/15384101.2014.980647

Cite this

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title = "Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication",

abstract = "The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as cells pass through late G1 and early S phase, identifying a brief window when MCM2 becomes transiently attached to the nuclear-matrix. The data distinguish 3 states that correspond to loose association with chromatin prior to DNA replication, transient highly stable binding to the nuclear-matrix coincident with initiation, and a post-initiation phase when MCM2 remains tightly associated with chromatin but not the nuclear-matrix. The data suggests that functional MCM complex loading takes place at the nuclear-matrix.",

keywords = "Cell cycle, DNA replication, Inititation, Minichromosome maintenance complex, MCM2-7, Nuclear matrix, NM, MCM, Minichromosome Maintenance",

author = "Hesketh, {Emma L} and Knight, {John RP} and Wilson, {Rosemary HC} and Chong, {James PJ} and Dawn Coverley",

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T1 - Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication

AU - Hesketh, Emma L

AU - Knight, John RP

AU - Wilson, Rosemary HC

AU - Chong, James PJ

AU - Coverley, Dawn

PY - 2015/2/6

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AB - The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as cells pass through late G1 and early S phase, identifying a brief window when MCM2 becomes transiently attached to the nuclear-matrix. The data distinguish 3 states that correspond to loose association with chromatin prior to DNA replication, transient highly stable binding to the nuclear-matrix coincident with initiation, and a post-initiation phase when MCM2 remains tightly associated with chromatin but not the nuclear-matrix. The data suggests that functional MCM complex loading takes place at the nuclear-matrix.

KW - Cell cycle

KW - DNA replication

KW - Inititation

KW - Minichromosome maintenance complex

KW - MCM2-7

KW - Nuclear matrix

KW - NM

KW - MCM

KW - Minichromosome Maintenance

U2 - 10.4161/15384101.2014.980647

DO - 10.4161/15384101.2014.980647

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