Triple bioorthogonal ligation strategy for simultaneous labeling of multiple enzymatic activities

TY - JOUR

T1 - Triple bioorthogonal ligation strategy for simultaneous labeling of multiple enzymatic activities

AU - Willems, Lianne I.

AU - Li, Nan

AU - Florea, Bogdan I.

AU - Ruben, Mark

AU - Van Der Marel, Gijsbert A.

AU - Overkleeft, Herman S.

PY - 2012/4/27

Y1 - 2012/4/27

N2 - Three at the same time: A ligation strategy combining tetrazine-norbornene cycloaddition, Staudinger-Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.

AB - Three at the same time: A ligation strategy combining tetrazine-norbornene cycloaddition, Staudinger-Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.

KW - activity-based profiling

KW - bioorthogonal reactions

KW - click chemistry

KW - cycloadditions

KW - tetrazines

UR - http://www.scopus.com/inward/record.url?scp=84860187002&partnerID=8YFLogxK

U2 - 10.1002/anie.201200923

DO - 10.1002/anie.201200923

M3 - Article

C2 - 22438272

AN - SCOPUS:84860187002

VL - 51

SP - 4431

EP - 4434

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

SN - 1433-7851

IS - 18

ER -