Three at the same time: A ligation strategy combining tetrazine-norbornene cycloaddition, Staudinger-Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.
|Number of pages||4|
|Journal||Angewandte Chemie International Edition|
|Publication status||Published - 27 Apr 2012|
- activity-based profiling
- bioorthogonal reactions
- click chemistry