Triple bioorthogonal ligation strategy for simultaneous labeling of multiple enzymatic activities

Lianne I. Willems*, Nan Li, Bogdan I. Florea, Mark Ruben, Gijsbert A. Van Der Marel, Herman S. Overkleeft

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Three at the same time: A ligation strategy combining tetrazine-norbornene cycloaddition, Staudinger-Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.

Original languageEnglish
Pages (from-to)4431-4434
Number of pages4
JournalAngewandte Chemie International Edition
Issue number18
Publication statusPublished - 27 Apr 2012


  • activity-based profiling
  • bioorthogonal reactions
  • click chemistry
  • cycloadditions
  • tetrazines

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