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From the same journal

Triple bioorthogonal ligation strategy for simultaneous labeling of multiple enzymatic activities

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Lianne I. Willems
  • Nan Li
  • Bogdan I. Florea
  • Mark Ruben
  • Gijsbert A. Van Der Marel
  • Herman S. Overkleeft

Department/unit(s)

Publication details

JournalAngewandte Chemie International Edition
DatePublished - 27 Apr 2012
Issue number18
Volume51
Number of pages4
Pages (from-to)4431-4434
Original languageEnglish

Abstract

Three at the same time: A ligation strategy combining tetrazine-norbornene cycloaddition, Staudinger-Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.

    Research areas

  • activity-based profiling, bioorthogonal reactions, click chemistry, cycloadditions, tetrazines

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