Two HlyIIR dimers bind to a long perfect inverted repeat in the operator of the hemolysin II gene from Bacillus cereus

Ekaterina A. Rodikova; Oleg V. Kovalevskiy; Sergey G. Mayorov; Zhanna I. Budarina; Victor V. Marchenkov; Bogdan S. Melnik; Andrew P. Leech; Dmitri V. Nikitin; Michael G. Shlyapnikov; Alexander S. Solonin

doi:10.1016/j.febslet.2007.02.035

Two HlyIIR dimers bind to a long perfect inverted repeat in the operator of the hemolysin II gene from Bacillus cereus

Ekaterina A. Rodikova, Oleg V. Kovalevskiy, Sergey G. Mayorov, Zhanna I. Budarina, Victor V. Marchenkov, Bogdan S. Melnik, Andrew P. Leech, Dmitri V. Nikitin, Michael G. Shlyapnikov, Alexander S. Solonin

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

HlyIIR is a negative transcriptional regulator of hemolysin II gene from B. cereus. It binds to a long DNA perfect inverted repeat (44 bp) located upstream the hlyll gene. Here we show that HlyIIR is dimeric in solution and in bacterial cells. No protein-protein interactions between dimers and no significant modification of target DNA conformation upon complex formation were observed. Two HlyIIR dimers were found to bind to native operator independently with Kd level in the nanomolar range. The minimal HlyIIR binding site was identified as a half of the long DNA perfect inverted repeat. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	1190-1196
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	6
DOIs	https://doi.org/10.1016/j.febslet.2007.02.035
Publication status	Published - 20 Mar 2007

Keywords

TetR family
DNA-binding
transcriptional regulator
CONFORMATIONAL-CHANGES
PROTEIN-DNA
THURINGIENSIS
REPRESSOR
SEQUENCE
ANTHRACIS
FAMILY
TOXINS
ASSAY

Access to Document

10.1016/j.febslet.2007.02.035

Cite this

Rodikova, E. A., Kovalevskiy, O. V., Mayorov, S. G., Budarina, Z. I., Marchenkov, V. V., Melnik, B. S., Leech, A. P., Nikitin, D. V., Shlyapnikov, M. G., & Solonin, A. S. (2007). Two HlyIIR dimers bind to a long perfect inverted repeat in the operator of the hemolysin II gene from Bacillus cereus. FEBS Letters, 581(6), 1190-1196. https://doi.org/10.1016/j.febslet.2007.02.035

@article{1779f41aa05e4c509494ef0011dd41a2,

title = "Two HlyIIR dimers bind to a long perfect inverted repeat in the operator of the hemolysin II gene from Bacillus cereus",

abstract = "HlyIIR is a negative transcriptional regulator of hemolysin II gene from B. cereus. It binds to a long DNA perfect inverted repeat (44 bp) located upstream the hlyll gene. Here we show that HlyIIR is dimeric in solution and in bacterial cells. No protein-protein interactions between dimers and no significant modification of target DNA conformation upon complex formation were observed. Two HlyIIR dimers were found to bind to native operator independently with Kd level in the nanomolar range. The minimal HlyIIR binding site was identified as a half of the long DNA perfect inverted repeat. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "TetR family, DNA-binding, transcriptional regulator, CONFORMATIONAL-CHANGES, PROTEIN-DNA, THURINGIENSIS, REPRESSOR, SEQUENCE, ANTHRACIS, FAMILY, TOXINS, ASSAY",

author = "Rodikova, {Ekaterina A.} and Kovalevskiy, {Oleg V.} and Mayorov, {Sergey G.} and Budarina, {Zhanna I.} and Marchenkov, {Victor V.} and Melnik, {Bogdan S.} and Leech, {Andrew P.} and Nikitin, {Dmitri V.} and Shlyapnikov, {Michael G.} and Solonin, {Alexander S.}",

year = "2007",

month = mar,

day = "20",

doi = "10.1016/j.febslet.2007.02.035",

language = "English",

volume = "581",

pages = "1190--1196",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "6",

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TY - JOUR

T1 - Two HlyIIR dimers bind to a long perfect inverted repeat in the operator of the hemolysin II gene from Bacillus cereus

AU - Rodikova, Ekaterina A.

AU - Kovalevskiy, Oleg V.

AU - Mayorov, Sergey G.

AU - Budarina, Zhanna I.

AU - Marchenkov, Victor V.

AU - Melnik, Bogdan S.

AU - Leech, Andrew P.

AU - Nikitin, Dmitri V.

AU - Shlyapnikov, Michael G.

AU - Solonin, Alexander S.

PY - 2007/3/20

Y1 - 2007/3/20

N2 - HlyIIR is a negative transcriptional regulator of hemolysin II gene from B. cereus. It binds to a long DNA perfect inverted repeat (44 bp) located upstream the hlyll gene. Here we show that HlyIIR is dimeric in solution and in bacterial cells. No protein-protein interactions between dimers and no significant modification of target DNA conformation upon complex formation were observed. Two HlyIIR dimers were found to bind to native operator independently with Kd level in the nanomolar range. The minimal HlyIIR binding site was identified as a half of the long DNA perfect inverted repeat. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - HlyIIR is a negative transcriptional regulator of hemolysin II gene from B. cereus. It binds to a long DNA perfect inverted repeat (44 bp) located upstream the hlyll gene. Here we show that HlyIIR is dimeric in solution and in bacterial cells. No protein-protein interactions between dimers and no significant modification of target DNA conformation upon complex formation were observed. Two HlyIIR dimers were found to bind to native operator independently with Kd level in the nanomolar range. The minimal HlyIIR binding site was identified as a half of the long DNA perfect inverted repeat. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KW - TetR family

KW - DNA-binding

KW - transcriptional regulator

KW - CONFORMATIONAL-CHANGES

KW - PROTEIN-DNA

KW - THURINGIENSIS

KW - REPRESSOR

KW - SEQUENCE

KW - ANTHRACIS

KW - FAMILY

KW - TOXINS

KW - ASSAY

U2 - 10.1016/j.febslet.2007.02.035

DO - 10.1016/j.febslet.2007.02.035

M3 - Article

SN - 0014-5793

VL - 581

SP - 1190

EP - 1196

JO - FEBS Letters

JF - FEBS Letters

IS - 6

ER -