Ulva prolifera polysaccharide enhances the root colonisation by Bacillus amyloliquefaciens strain Cas02

Depeng Chu, Xiaoqiang Wang, Jiajun Fan, Lijuan Peng, Jinchang Liang, Yanfen Zheng, Rui Wang, Xiangwei You, Changliang Jing, James Clark, Yiqiang Li*, Yuan Yuan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The application of biocontrol agent is important for the sustainable development of agriculture. Unsuccessful or limited colonisation by plant growth-promoting rhizobacteria (PGPR) has become an important constraint factor for their commercial application. Here, we report that Ulva prolifera polysaccharide (UPP) promotes root colonisation by Bacillus amyloliquefaciens strain Cas02. UPP serves as an environmental signal for bacterial biofilm formation and its glucose residue is used as a carbon source for the synthesis of the exopolysaccharides and poly-gamma-glutamate present in biofilm matrix. Greenhouse experiments demonstrated that UPP could effectively enhance the root colonisation by Cas02 in both the bacterial population and survival time under natural semiarid soil conditions. Furthermore, the microbiome analysis also indicated the promoted colonisation by Cas02, as well as the improved bacterial rhizosphere community structure, after combined treatment of UPP and Cas02. This study provides a practical approach to improve the biocontrol agent with seaweed polysaccharides.

Original languageEnglish
Article number120171
Number of pages10
JournalCARBOHYDRATE POLYMERS
Volume299
Early online date5 Oct 2022
DOIs
Publication statusPublished - 1 Jan 2023

Bibliographical note

Funding Information:
This work was supported by the Doctor Foundation of Shandong ( ZR2021QD062 ), by the International Foundation of Tobacco Research Institute of CAAS ( IFI202103 ), by the Major Tobacco Science and Technology Projects ( 110202101054(LS-14), 201906 ), by the Agricultural Science and Technology Innovation Program of China ( ASTIP-TRIC04 , ASTIP-TRIC06 ).

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

  • Bacillus amyloliquefaciens
  • Biofilm
  • Rhizosphere microbiome
  • Root colonisation
  • Ulva prolifera polysaccharide

Cite this