Understanding hematopoiesis from a single-cell standpoint.

KD Kokkaliaris; D Lucas; I Beerman; DG Kent; L Perié

doi:10.1016/j.exphem.2016.03.003

Understanding hematopoiesis from a single-cell standpoint.

KD Kokkaliaris, D Lucas, I Beerman, DG Kent, L Perié

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The cellular diversity of the hematopoietic system has been extensively studied, and a plethora of cell surface markers have been used to discriminate and prospectively purify different blood cell types. However, even within phenotypically identical fractions of hematopoietic stem and progenitor cells or lineage-restricted progenitors, significant functional heterogeneity is observed when single cells are analyzed. To address these challenges, researchers are now using techniques to follow single cells and their progeny to improve our understanding of the underlying functional heterogeneity. On November 19, 2015, Dr. David Kent and Dr. Leïla Perié, two emerging young group leaders, presented their recent efforts to dissect the functional properties of individual cells with a webinar series organized by the International Society for Experimental Hematology. Here, we provide a summary of the presented methods for cell labeling and clonal tracking and discuss how these different techniques have been employed to study hematopoiesis.

Original language	English
Pages (from-to)	447-450
Number of pages	4
Journal	Experimental hematology
Volume	44
Issue number	6
DOIs	https://doi.org/10.1016/j.exphem.2016.03.003
Publication status	Published - 1 Jun 2016

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10.1016/j.exphem.2016.03.003

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title = "Understanding hematopoiesis from a single-cell standpoint.",

abstract = "The cellular diversity of the hematopoietic system has been extensively studied, and a plethora of cell surface markers have been used to discriminate and prospectively purify different blood cell types. However, even within phenotypically identical fractions of hematopoietic stem and progenitor cells or lineage-restricted progenitors, significant functional heterogeneity is observed when single cells are analyzed. To address these challenges, researchers are now using techniques to follow single cells and their progeny to improve our understanding of the underlying functional heterogeneity. On November 19, 2015, Dr. David Kent and Dr. Le{\"i}la Peri{\'e}, two emerging young group leaders, presented their recent efforts to dissect the functional properties of individual cells with a webinar series organized by the International Society for Experimental Hematology. Here, we provide a summary of the presented methods for cell labeling and clonal tracking and discuss how these different techniques have been employed to study hematopoiesis.",

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AU - Kokkaliaris, KD

AU - Lucas, D

AU - Beerman, I

AU - Kent, DG

AU - Perié, L

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Y1 - 2016/6/1

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AB - The cellular diversity of the hematopoietic system has been extensively studied, and a plethora of cell surface markers have been used to discriminate and prospectively purify different blood cell types. However, even within phenotypically identical fractions of hematopoietic stem and progenitor cells or lineage-restricted progenitors, significant functional heterogeneity is observed when single cells are analyzed. To address these challenges, researchers are now using techniques to follow single cells and their progeny to improve our understanding of the underlying functional heterogeneity. On November 19, 2015, Dr. David Kent and Dr. Leïla Perié, two emerging young group leaders, presented their recent efforts to dissect the functional properties of individual cells with a webinar series organized by the International Society for Experimental Hematology. Here, we provide a summary of the presented methods for cell labeling and clonal tracking and discuss how these different techniques have been employed to study hematopoiesis.

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