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Understanding hematopoiesis from a single-cell standpoint.

Research output: Contribution to journalArticlepeer-review


  • KD Kokkaliaris
  • D Lucas
  • I Beerman
  • DG Kent
  • L Perié


Publication details

JournalExperimental hematology
DatePublished - 1 Jun 2016
Issue number6
Number of pages4
Pages (from-to)447-450
Original languageEnglish


The cellular diversity of the hematopoietic system has been extensively studied, and a plethora of cell surface markers have been used to discriminate and prospectively purify different blood cell types. However, even within phenotypically identical fractions of hematopoietic stem and progenitor cells or lineage-restricted progenitors, significant functional heterogeneity is observed when single cells are analyzed. To address these challenges, researchers are now using techniques to follow single cells and their progeny to improve our understanding of the underlying functional heterogeneity. On November 19, 2015, Dr. David Kent and Dr. Leïla Perié, two emerging young group leaders, presented their recent efforts to dissect the functional properties of individual cells with a webinar series organized by the International Society for Experimental Hematology. Here, we provide a summary of the presented methods for cell labeling and clonal tracking and discuss how these different techniques have been employed to study hematopoiesis.

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