By the same authors

From the same journal

Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • S. Chapel-Fernandes
  • F. Jordier
  • F. Lauro
  • N. Maitland
  • J. Chiaroni
  • P. de Micco
  • P. Mannoni
  • C. Bagnis

Department/unit(s)

Publication details

JournalCancer gene therapy
DatePublished - Oct 2006
Issue number10
Volume13
Number of pages11
Pages (from-to)919-929
Original languageEnglish

Abstract

\Composite promoters combining the prostate-specific antigen (PSA) enhancer core element with promoter elements derived from gene coding for human prostate-specific transglutaminase gene, prostate-specific membrane antigen gene, prostate-specific antigen, rat probasin or phosphoglycerate kinase were characterized for their ability to specifically express the enhanced green fluorescent protein (EGFP) gene in prostate versus non-prostate cancer cell lines when transferred with a human immunodeficiency virus-1-based lentiviral vector. By themselves minimal proximal promoter elements were found to inefficiently promote relevant tissue-specific expression; in all the vectors tested, addition of the PSA enhancer core element markedly improved EGFP expression in LnCaP, a cancer prostate cell line used as a model for prostate cancer. The composite promoter was inactive in HuH7,a hepatocarcinoma cell line used as a model of neighboring non-prostate cancer cells. Among the promoters tested, the combination of the PSA enhancer and the rat probasin promoter showed both high specificity and a strong EGFP expression. Neither a high viral input nor the presence of the cPPT/CTS sequence affected composite promoter behavior. Our data suggest that composite prostate-specific promoters constructed by combining key elements from various promoters can improve and/or confer tissue specific expression in a lentiviral vector context.

    Research areas

  • lentiviral vector, prostate cancer, specific expression, PSA, TISSUE-SPECIFIC EXPRESSION, TUMOR-ASSOCIATED NEOVASCULATURE, VIVO GENE DELIVERY, CENTRAL DNA FLAP, MEMBRANE ANTIGEN, IN-VIVO, REGULATED EXPRESSION, PROBASIN GENE, EFFICIENT TRANSDUCTION, TRANSGLUTAMINASE GENE

Projects

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations