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Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context

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Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context. / Chapel-Fernandes, S.; Jordier, F.; Lauro, F.; Maitland, N.; Chiaroni, J.; de Micco, P.; Mannoni, P.; Bagnis, C.

In: Cancer gene therapy, Vol. 13, No. 10, 10.2006, p. 919-929.

Research output: Contribution to journalArticle

Harvard

Chapel-Fernandes, S, Jordier, F, Lauro, F, Maitland, N, Chiaroni, J, de Micco, P, Mannoni, P & Bagnis, C 2006, 'Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context', Cancer gene therapy, vol. 13, no. 10, pp. 919-929. https://doi.org/10.1038/sj.cgt.7700966

APA

Chapel-Fernandes, S., Jordier, F., Lauro, F., Maitland, N., Chiaroni, J., de Micco, P., ... Bagnis, C. (2006). Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context. Cancer gene therapy, 13(10), 919-929. https://doi.org/10.1038/sj.cgt.7700966

Vancouver

Chapel-Fernandes S, Jordier F, Lauro F, Maitland N, Chiaroni J, de Micco P et al. Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context. Cancer gene therapy. 2006 Oct;13(10):919-929. https://doi.org/10.1038/sj.cgt.7700966

Author

Chapel-Fernandes, S. ; Jordier, F. ; Lauro, F. ; Maitland, N. ; Chiaroni, J. ; de Micco, P. ; Mannoni, P. ; Bagnis, C. / Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context. In: Cancer gene therapy. 2006 ; Vol. 13, No. 10. pp. 919-929.

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@article{7b9dd8eace8e497197cded06b90337d9,
title = "Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context",
abstract = "\Composite promoters combining the prostate-specific antigen (PSA) enhancer core element with promoter elements derived from gene coding for human prostate-specific transglutaminase gene, prostate-specific membrane antigen gene, prostate-specific antigen, rat probasin or phosphoglycerate kinase were characterized for their ability to specifically express the enhanced green fluorescent protein (EGFP) gene in prostate versus non-prostate cancer cell lines when transferred with a human immunodeficiency virus-1-based lentiviral vector. By themselves minimal proximal promoter elements were found to inefficiently promote relevant tissue-specific expression; in all the vectors tested, addition of the PSA enhancer core element markedly improved EGFP expression in LnCaP, a cancer prostate cell line used as a model for prostate cancer. The composite promoter was inactive in HuH7,a hepatocarcinoma cell line used as a model of neighboring non-prostate cancer cells. Among the promoters tested, the combination of the PSA enhancer and the rat probasin promoter showed both high specificity and a strong EGFP expression. Neither a high viral input nor the presence of the cPPT/CTS sequence affected composite promoter behavior. Our data suggest that composite prostate-specific promoters constructed by combining key elements from various promoters can improve and/or confer tissue specific expression in a lentiviral vector context.",
keywords = "lentiviral vector, prostate cancer, specific expression, PSA, TISSUE-SPECIFIC EXPRESSION, TUMOR-ASSOCIATED NEOVASCULATURE, VIVO GENE DELIVERY, CENTRAL DNA FLAP, MEMBRANE ANTIGEN, IN-VIVO, REGULATED EXPRESSION, PROBASIN GENE, EFFICIENT TRANSDUCTION, TRANSGLUTAMINASE GENE",
author = "S. Chapel-Fernandes and F. Jordier and F. Lauro and N. Maitland and J. Chiaroni and {de Micco}, P. and P. Mannoni and C. Bagnis",
year = "2006",
month = "10",
doi = "10.1038/sj.cgt.7700966",
language = "English",
volume = "13",
pages = "919--929",
journal = "Cancer gene therapy",
issn = "0929-1903",
publisher = "Nature Publishing Group",
number = "10",

}

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TY - JOUR

T1 - Use of the PSA enhancer core element to modulate the expression of prostate- and non-prostate-specific basal promoters in a lentiviral vector context

AU - Chapel-Fernandes, S.

AU - Jordier, F.

AU - Lauro, F.

AU - Maitland, N.

AU - Chiaroni, J.

AU - de Micco, P.

AU - Mannoni, P.

AU - Bagnis, C.

PY - 2006/10

Y1 - 2006/10

N2 - \Composite promoters combining the prostate-specific antigen (PSA) enhancer core element with promoter elements derived from gene coding for human prostate-specific transglutaminase gene, prostate-specific membrane antigen gene, prostate-specific antigen, rat probasin or phosphoglycerate kinase were characterized for their ability to specifically express the enhanced green fluorescent protein (EGFP) gene in prostate versus non-prostate cancer cell lines when transferred with a human immunodeficiency virus-1-based lentiviral vector. By themselves minimal proximal promoter elements were found to inefficiently promote relevant tissue-specific expression; in all the vectors tested, addition of the PSA enhancer core element markedly improved EGFP expression in LnCaP, a cancer prostate cell line used as a model for prostate cancer. The composite promoter was inactive in HuH7,a hepatocarcinoma cell line used as a model of neighboring non-prostate cancer cells. Among the promoters tested, the combination of the PSA enhancer and the rat probasin promoter showed both high specificity and a strong EGFP expression. Neither a high viral input nor the presence of the cPPT/CTS sequence affected composite promoter behavior. Our data suggest that composite prostate-specific promoters constructed by combining key elements from various promoters can improve and/or confer tissue specific expression in a lentiviral vector context.

AB - \Composite promoters combining the prostate-specific antigen (PSA) enhancer core element with promoter elements derived from gene coding for human prostate-specific transglutaminase gene, prostate-specific membrane antigen gene, prostate-specific antigen, rat probasin or phosphoglycerate kinase were characterized for their ability to specifically express the enhanced green fluorescent protein (EGFP) gene in prostate versus non-prostate cancer cell lines when transferred with a human immunodeficiency virus-1-based lentiviral vector. By themselves minimal proximal promoter elements were found to inefficiently promote relevant tissue-specific expression; in all the vectors tested, addition of the PSA enhancer core element markedly improved EGFP expression in LnCaP, a cancer prostate cell line used as a model for prostate cancer. The composite promoter was inactive in HuH7,a hepatocarcinoma cell line used as a model of neighboring non-prostate cancer cells. Among the promoters tested, the combination of the PSA enhancer and the rat probasin promoter showed both high specificity and a strong EGFP expression. Neither a high viral input nor the presence of the cPPT/CTS sequence affected composite promoter behavior. Our data suggest that composite prostate-specific promoters constructed by combining key elements from various promoters can improve and/or confer tissue specific expression in a lentiviral vector context.

KW - lentiviral vector

KW - prostate cancer

KW - specific expression

KW - PSA

KW - TISSUE-SPECIFIC EXPRESSION

KW - TUMOR-ASSOCIATED NEOVASCULATURE

KW - VIVO GENE DELIVERY

KW - CENTRAL DNA FLAP

KW - MEMBRANE ANTIGEN

KW - IN-VIVO

KW - REGULATED EXPRESSION

KW - PROBASIN GENE

KW - EFFICIENT TRANSDUCTION

KW - TRANSGLUTAMINASE GENE

U2 - 10.1038/sj.cgt.7700966

DO - 10.1038/sj.cgt.7700966

M3 - Article

VL - 13

SP - 919

EP - 929

JO - Cancer gene therapy

JF - Cancer gene therapy

SN - 0929-1903

IS - 10

ER -