Validation of a new method for immobilising kinetoplastid parasites for live cell imaging

Helen P. Price; Lorna MacLean; Joanne Marrison; Peter John O'Toole; Deborah F. Smith

doi:10.1016/j.molbiopara.2009.09.008

Validation of a new method for immobilising kinetoplastid parasites for live cell imaging

Helen P. Price, Lorna MacLean, Joanne Marrison, Peter John O'Toole, Deborah F. Smith

Research output: Contribution to journal › Article › peer-review

Abstract

The kinetoplastid parasites are responsible for three of the ten most neglected tropical diseases as classified by the WHO. Recent advances in molecular and cellular analyses have allowed rapid progress in our understanding of the biology of these lethal pathogens. in this study we validate a new method for immobilising Trypanosoma brucei and Leishmania major parasites while maintaining a high level of viability. This allows reproducible live cell imaging of these highly motile organisms, thus enabling a full complement of advanced microscopic techniques to be utilised to better understand these pathogenic species. (C) 2009 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	66-69
Number of pages	4
Journal	MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume	169
Issue number	1
DOIs	https://doi.org/10.1016/j.molbiopara.2009.09.008
Publication status	Published - Jan 2010

Keywords

Trypanosoma brucei
Leishmania major
Live cell imaging
FRAP
TRYPANOSOMA-BRUCEI
LEISHMANIA

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10.1016/j.molbiopara.2009.09.008

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abstract = "The kinetoplastid parasites are responsible for three of the ten most neglected tropical diseases as classified by the WHO. Recent advances in molecular and cellular analyses have allowed rapid progress in our understanding of the biology of these lethal pathogens. in this study we validate a new method for immobilising Trypanosoma brucei and Leishmania major parasites while maintaining a high level of viability. This allows reproducible live cell imaging of these highly motile organisms, thus enabling a full complement of advanced microscopic techniques to be utilised to better understand these pathogenic species. (C) 2009 Elsevier B.V. All rights reserved.",

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AU - Smith, Deborah F.

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AB - The kinetoplastid parasites are responsible for three of the ten most neglected tropical diseases as classified by the WHO. Recent advances in molecular and cellular analyses have allowed rapid progress in our understanding of the biology of these lethal pathogens. in this study we validate a new method for immobilising Trypanosoma brucei and Leishmania major parasites while maintaining a high level of viability. This allows reproducible live cell imaging of these highly motile organisms, thus enabling a full complement of advanced microscopic techniques to be utilised to better understand these pathogenic species. (C) 2009 Elsevier B.V. All rights reserved.

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Validation of a new method for immobilising kinetoplastid parasites for live cell imaging

Abstract

Keywords

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