Validation of swabs as a non-destructive and relatively non-invasive DNA sampling method in fish

A. L. Le Vin; A. Adam; A. Tedder; K. E. Arnold; B. K. Mable

doi:10.1111/j.1755-0998.2010.02909.x

Validation of swabs as a non-destructive and relatively non-invasive DNA sampling method in fish

A. L. Le Vin, A. Adam, A. Tedder, K. E. Arnold, B. K. Mable

Environment and Geography

Research output: Contribution to journal › Article › peer-review

Abstract

Non-destructive methods of collecting DNA from small fish species can be problematic, as fin clips can potentially affect behaviour or survivorship in the wild. Swabbing body mucus may provide a less invasive method of DNA collection. However, risk of contamination from other individuals in high density groups could give erroneous genotyping results. We compared multilocus microsatellite genotypes from the same individuals when collected at low and high density and compared this with fin clips. We found no differences between these categories, with a genotyping error rate of 0.42%, validating the use of body mucus swabbing for DNA collection in fish.

Original language	English
Pages (from-to)	107-109
Number of pages	3
Journal	Molecular ecology resources
Volume	11
Issue number	1
DOIs	https://doi.org/10.1111/j.1755-0998.2010.02909.x
Publication status	Published - Jan 2011

Keywords

contamination
fin clip
fish body mucus
Neolamprologus pulcher
SURVIVAL
TROUT
CONSERVATION
ANESTHESIA
MARKERS

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10.1111/j.1755-0998.2010.02909.x

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abstract = "Non-destructive methods of collecting DNA from small fish species can be problematic, as fin clips can potentially affect behaviour or survivorship in the wild. Swabbing body mucus may provide a less invasive method of DNA collection. However, risk of contamination from other individuals in high density groups could give erroneous genotyping results. We compared multilocus microsatellite genotypes from the same individuals when collected at low and high density and compared this with fin clips. We found no differences between these categories, with a genotyping error rate of 0.42%, validating the use of body mucus swabbing for DNA collection in fish.",

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AU - Le Vin, A. L.

AU - Adam, A.

AU - Tedder, A.

AU - Arnold, K. E.

AU - Mable, B. K.

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KW - contamination

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KW - fish body mucus

KW - Neolamprologus pulcher

KW - SURVIVAL

KW - TROUT

KW - CONSERVATION

KW - ANESTHESIA

KW - MARKERS

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JO - Molecular ecology resources

JF - Molecular ecology resources

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Validation of swabs as a non-destructive and relatively non-invasive DNA sampling method in fish

Abstract

Keywords

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