TY - JOUR
T1 - Vapyrin, a gene essential for intracellular progression of arbuscular mycorrhizal symbiosis, is also essential for infection by rhizobia in the nodule symbiosis of Medicago truncatula
AU - Murray, Jeremy D.
AU - Muni, RajaSekhara Reddy Duvvuru
AU - Torres-Jerez, Ivone
AU - Tang, Yuhong
AU - Allen, Stacy
AU - Andriankaja, Megan
AU - Li, Guangming
AU - Laxmi, Ashverya
AU - Cheng, Xiaofei
AU - Wen, Jiangqi
AU - Vaughan, David
AU - Schultze, Michael
AU - Sun, Jongho
AU - Charpentier, Myriam
AU - Oldroyd, Giles
AU - Tadege, Million
AU - Ratet, Pascal
AU - Mysore, Kirankumar S.
AU - Chen, Rujin
AU - Udvardi, Michael K.
PY - 2011/1
Y1 - 2011/1
N2 - P>Intracellular invasion of root cells is required for the establishment of successful endosymbioses in legumes of both arbuscular mycorrhizal (AM) fungi and rhizobial bacteria. In both interactions a requirement for successful entry is the activation of a common signalling pathway that includes five genes required to generate calcium oscillations and two genes required for the perception of the calcium response. Recently, it has been discovered that in Medicago truncatula, the Vapyrin (VPY) gene is essential for the establishment of the arbuscular mycorrhizal symbiosis. Here, we show by analyses of mutants that the same gene is also required for rhizobial colonization and nodulation. VPY encodes a protein featuring a Major Sperm Protein domain, typically featured on proteins involved in membrane trafficking and biogenesis, and a series of ankyrin repeats. Plants mutated in this gene have abnormal rhizobial infection threads and fewer nodules, and in the case of interactions with AM fungi, epidermal penetration defects and aborted arbuscule formation. Calcium spiking in root hairs in response to supplied Nod factors is intact in the vpy-1 mutant. This, and the elevation of VPY transcripts upon application of Nod factors which we show to be dependent on NFP, DMI1, and DMI3, indicates that VPY acts downstream of the common signalling pathway.
AB - P>Intracellular invasion of root cells is required for the establishment of successful endosymbioses in legumes of both arbuscular mycorrhizal (AM) fungi and rhizobial bacteria. In both interactions a requirement for successful entry is the activation of a common signalling pathway that includes five genes required to generate calcium oscillations and two genes required for the perception of the calcium response. Recently, it has been discovered that in Medicago truncatula, the Vapyrin (VPY) gene is essential for the establishment of the arbuscular mycorrhizal symbiosis. Here, we show by analyses of mutants that the same gene is also required for rhizobial colonization and nodulation. VPY encodes a protein featuring a Major Sperm Protein domain, typically featured on proteins involved in membrane trafficking and biogenesis, and a series of ankyrin repeats. Plants mutated in this gene have abnormal rhizobial infection threads and fewer nodules, and in the case of interactions with AM fungi, epidermal penetration defects and aborted arbuscule formation. Calcium spiking in root hairs in response to supplied Nod factors is intact in the vpy-1 mutant. This, and the elevation of VPY transcripts upon application of Nod factors which we show to be dependent on NFP, DMI1, and DMI3, indicates that VPY acts downstream of the common signalling pathway.
UR - http://www.scopus.com/inward/record.url?scp=78651400692&partnerID=8YFLogxK
U2 - 10.1111/j.1365-313X.2010.04415.x
DO - 10.1111/j.1365-313X.2010.04415.x
M3 - Article
SN - 0960-7412
VL - 65
SP - 244
EP - 252
JO - Plant Journal
JF - Plant Journal
IS - 2
ER -