Variation of terpene alkaloids in Daphniphyllum macropodum across plants and tissues

TY - JOUR

T1 - Variation of terpene alkaloids in Daphniphyllum macropodum across plants and tissues

AU - Eljounaidi, Kaouthar

AU - Radzikowska, Barbara A

AU - Whitehead, Caragh B

AU - Taylor, Danielle J

AU - Conde, Susana

AU - Davis, William

AU - Dowle, Adam A

AU - Langer, Swen

AU - James, Sally

AU - Unsworth, William P

AU - Ezer, Daphne

AU - Larson, Tony R

AU - Lichman, Benjamin R

N1 - Publisher Copyright: © 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.

PY - 2024/7

Y1 - 2024/7

N2 - Daphniphyllum macropodum produces alkaloids that are structurally complex with polycyclic, stereochemically rich carbon skeletons. Understanding how these compounds are formed by the plant may enable exploration of their biological function and bioactivities. We employed multiple metabolomics techniques, including a workflow to annotate compounds in the absence of standards, to compare alkaloid content across plants and tissues. Different alkaloid structural types were found to have distinct distributions between genotypes, between tissues and within tissues. Alkaloid structural types also showed different isotope labelling enrichments that matched their biosynthetic relationships. The work suggests that mevalonate derived 30-carbon alkaloids are formed in the phloem region before their conversion to 22-carbon alkaloids which accumulate in the epidermis. This sets the stage for further investigation into the biosynthetic pathway.

AB - Daphniphyllum macropodum produces alkaloids that are structurally complex with polycyclic, stereochemically rich carbon skeletons. Understanding how these compounds are formed by the plant may enable exploration of their biological function and bioactivities. We employed multiple metabolomics techniques, including a workflow to annotate compounds in the absence of standards, to compare alkaloid content across plants and tissues. Different alkaloid structural types were found to have distinct distributions between genotypes, between tissues and within tissues. Alkaloid structural types also showed different isotope labelling enrichments that matched their biosynthetic relationships. The work suggests that mevalonate derived 30-carbon alkaloids are formed in the phloem region before their conversion to 22-carbon alkaloids which accumulate in the epidermis. This sets the stage for further investigation into the biosynthetic pathway.

KW - alkaloids

KW - biosynthesis

KW - Daphniphyllum

KW - metabolomics

KW - specialised metabolism

UR - http://www.scopus.com/inward/record.url?scp=85193501164&partnerID=8YFLogxK

U2 - 10.1111/nph.19814

DO - 10.1111/nph.19814

M3 - Article

C2 - 38757546

SN - 0028-646X

VL - 243

SP - 299

EP - 313

JO - The New phytologist

JF - The New phytologist

IS - 1

ER -