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From the same journal

Visualisation of lymphoid organ development

Research output: Contribution to journalArticle

Published copy (DOI)

Author(s)

  • Henrique Veiga-Fernandes
  • Katie Foster
  • Amisha Patel
  • Mark Coles
  • Dimitris Kioussis

Department/unit(s)

Publication details

JournalMethods in molecular biology (Clifton, N.J.)
DatePublished - 2010
Volume616
Number of pages19
Pages (from-to)161-79
Original languageEnglish

Abstract

This chapter provides information on imaging tools that can be employed to visualise and study lymphoid organ development. We focus on the use of genetically modified mouse models that take advantage of fluorescent protein expression in discrete cell populations, thus allowing live cell imaging during lymphoid organogenesis. We discuss approaches that allow characterisation of the cell types involved in the formation of lymphoid organs, including (i) functional assays in explant organ cultures and (ii) high-resolution whole-mount immunostaining methods, which are useful for the characterisation of specific cell populations in the context of the whole developing organ.

    Research areas

  • Animals, Cell Lineage, Cell Survival, Dissection, Embryo, Mammalian, Humans, Imaging, Three-Dimensional, Luminescent Proteins, Lymphatic System, Mice, Models, Animal, Organogenesis, Staining and Labeling

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