Visualization of hidden epitopes at the inactive X chromosome

Emma R. Stewart*, Dawn Coverley

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


Immunodetection of nuclear antigens is often complicated by epitope masking, so that proteins known to function in the nucleus are sometimes not easily detected at their sites of action. Moreover, protein populations that are detected before unmasking can be very different to those seen after removal of nucleic acids. This is particularly true for components of the nuclear matrix, including those known to function at the inactive X chromosome. Here we describe an unmasking protocol that reveals previously undetected proteins at the inactive X chromosome in mouse fibroblasts.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • CIZ1
  • Immunofluorescence
  • Inactive X chromosome
  • Nuclear matrix
  • SAF-A
  • Unmasking nuclear proteins

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