By the same authors

Visualization of hidden epitopes at the inactive X chromosome

Research output: Chapter in Book/Report/Conference proceedingChapter

Published copy (DOI)



Publication details

Title of host publicationMethods in Molecular Biology
DatePublished - 2018
Number of pages10
PublisherHumana Press Inc.
Original languageEnglish

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


Immunodetection of nuclear antigens is often complicated by epitope masking, so that proteins known to function in the nucleus are sometimes not easily detected at their sites of action. Moreover, protein populations that are detected before unmasking can be very different to those seen after removal of nucleic acids. This is particularly true for components of the nuclear matrix, including those known to function at the inactive X chromosome. Here we describe an unmasking protocol that reveals previously undetected proteins at the inactive X chromosome in mouse fibroblasts.

    Research areas

  • CIZ1, Immunofluorescence, Inactive X chromosome, Nuclear matrix, SAF-A, Unmasking nuclear proteins

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