Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase

Yuan He; Matthew S. Macauley; Keith A. Stubbs; David J. Vocadlo; Gideon J. Davies

doi:10.1021/ja9086769

Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase

Yuan He, Matthew S. Macauley, Keith A. Stubbs, David J. Vocadlo, Gideon J. Davies

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response. and transcriptional regulation Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) Using, a substrate-assisted catalytic mechanism. Here we define the reaction coordinate Using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.

Original language	English
Pages (from-to)	1807-1809
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	132
Issue number	6
DOIs	https://doi.org/10.1021/ja9086769
Publication status	Published - 17 Feb 2010

Keywords

STRUCTURAL INSIGHTS
CATALYTIC MECHANISM
PHOSPHORYLATION
GLCNACYLATION
INHIBITORS
DISEASE
MOTION
TAU

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10.1021/ja9086769

http://pubs.acs.org/doi/abs/10.1021/ja9086769

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title = "Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase",

abstract = "N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response. and transcriptional regulation Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) Using, a substrate-assisted catalytic mechanism. Here we define the reaction coordinate Using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.",

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AU - He, Yuan

AU - Macauley, Matthew S.

AU - Stubbs, Keith A.

AU - Vocadlo, David J.

AU - Davies, Gideon J.

PY - 2010/2/17

Y1 - 2010/2/17

N2 - N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response. and transcriptional regulation Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) Using, a substrate-assisted catalytic mechanism. Here we define the reaction coordinate Using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.

AB - N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response. and transcriptional regulation Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) Using, a substrate-assisted catalytic mechanism. Here we define the reaction coordinate Using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.

KW - STRUCTURAL INSIGHTS

KW - CATALYTIC MECHANISM

KW - PHOSPHORYLATION

KW - GLCNACYLATION

KW - INHIBITORS

KW - DISEASE

KW - MOTION

KW - TAU

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DO - 10.1021/ja9086769

M3 - Article

SN - 0002-7863

VL - 132

SP - 1807

EP - 1809

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 6

ER -

Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase

Abstract

Keywords

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