Wnt-dependent osteogenic commitment of bone marrow stromal cells using a novel GSK3β inhibitor

David A Cook, Simon W Fellgett, Mary E Pownall, Patrick J O'Shea, Paul G Genever

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Bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) can differentiate into multiple lineages including osteogenic and adipogenic cells. Wnt signalling has been implicated in controlling BMSC fate, but the mechanisms are unclear and apparently conflicting data exist. Here we show that a novel glycogen synthase kinase 3β inhibitor, AR28, is a potent activator of canonical Wnt signalling using in vitro β-catenin translocation studies and TCF-reporter assays. In vivo, AR28 induced characteristic axis duplication and secondary regions of chordin expression in Xenopus laevis embryos. Using human BMSCs grown in adipogenic medium, we confirmed that AR28-mediated Wnt signalling caused a significant (p
Original languageEnglish
Pages (from-to)415-427
JournalStem Cell Research
Issue number2
Publication statusPublished - 1 Mar 2014

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