X-ray diffraction from DNA fibres under tension

R.J. Greenall; C. Nave; W. Fuller

doi:10.1006/jmbi.2000.4325

X-ray diffraction from DNA fibres under tension

R.J. Greenall, C. Nave, W. Fuller

Physics

Research output: Contribution to journal › Article › peer-review

Abstract

When DNA fibres are stretched during drying, the polymer undergoes a conformational transition. We present quantitative results from X-ray diffraction studies on such fibres held at various ambient relative humidities. These indicate that the molecules are arranged in arrays which are crystalline in projection down the fibre axis. The packing can be explained in terms of a hexagonal cell with a lattice parameter, a, of 13 Å which varies with humidity. The patterns contain meridional intensities at 1/3.4 Å-1 and 1/6.5 Å-1, a strong off-meridional intensity at Z=1/5.6 Å-1 and diffuse scatter at Z=1/28 Å-1.

Original language	English
Pages (from-to)	669-672
Number of pages	3
Journal	Journal of Molecular Biology
Volume	305
Issue number	4
DOIs	https://doi.org/10.1006/jmbi.2000.4325
Publication status	Published - Jan 2001

Keywords

DNA
conformation
polymorphism
fibre diffraction
SUPERCOILED DNA
STRETCHING DNA
SINGLE
ELASTICITY
MOLECULES
FORCES

Access to Document

10.1006/jmbi.2000.4325

http://dx.doi.org/10.1006/jmbi.2000.4325

Cite this

@article{133dd3434cf64c8e8255f4b18be54d09,

title = "X-ray diffraction from DNA fibres under tension",

abstract = "When DNA fibres are stretched during drying, the polymer undergoes a conformational transition. We present quantitative results from X-ray diffraction studies on such fibres held at various ambient relative humidities. These indicate that the molecules are arranged in arrays which are crystalline in projection down the fibre axis. The packing can be explained in terms of a hexagonal cell with a lattice parameter, a, of 13 {\AA} which varies with humidity. The patterns contain meridional intensities at 1/3.4 {\AA}-1 and 1/6.5 {\AA}-1, a strong off-meridional intensity at Z=1/5.6 {\AA}-1 and diffuse scatter at Z=1/28 {\AA}-1.",

keywords = "DNA, conformation, polymorphism, fibre diffraction, SUPERCOILED DNA, STRETCHING DNA, SINGLE, ELASTICITY, MOLECULES, FORCES",

author = "R.J. Greenall and C. Nave and W. Fuller",

year = "2001",

month = jan,

doi = "10.1006/jmbi.2000.4325",

language = "English",

volume = "305",

pages = "669--672",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

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T1 - X-ray diffraction from DNA fibres under tension

AU - Greenall, R.J.

AU - Nave, C.

AU - Fuller, W.

PY - 2001/1

Y1 - 2001/1

N2 - When DNA fibres are stretched during drying, the polymer undergoes a conformational transition. We present quantitative results from X-ray diffraction studies on such fibres held at various ambient relative humidities. These indicate that the molecules are arranged in arrays which are crystalline in projection down the fibre axis. The packing can be explained in terms of a hexagonal cell with a lattice parameter, a, of 13 Å which varies with humidity. The patterns contain meridional intensities at 1/3.4 Å-1 and 1/6.5 Å-1, a strong off-meridional intensity at Z=1/5.6 Å-1 and diffuse scatter at Z=1/28 Å-1.

AB - When DNA fibres are stretched during drying, the polymer undergoes a conformational transition. We present quantitative results from X-ray diffraction studies on such fibres held at various ambient relative humidities. These indicate that the molecules are arranged in arrays which are crystalline in projection down the fibre axis. The packing can be explained in terms of a hexagonal cell with a lattice parameter, a, of 13 Å which varies with humidity. The patterns contain meridional intensities at 1/3.4 Å-1 and 1/6.5 Å-1, a strong off-meridional intensity at Z=1/5.6 Å-1 and diffuse scatter at Z=1/28 Å-1.

KW - DNA

KW - conformation

KW - polymorphism

KW - fibre diffraction

KW - SUPERCOILED DNA

KW - STRETCHING DNA

KW - SINGLE

KW - ELASTICITY

KW - MOLECULES

KW - FORCES

U2 - 10.1006/jmbi.2000.4325

DO - 10.1006/jmbi.2000.4325

M3 - Article

SN - 0022-2836

VL - 305

SP - 669

EP - 672

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -